High performance liquid chromatographic separation of stereoisomeric bile acids as their UV-sensitive esters |
| |
Authors: | Takashi Iida Yasuo Ohnuki Frederic C Chang Junichi Goto Toshio Nambara |
| |
Affiliation: | (1) College of Engineering, Nihon University, 963 Koriyama Fukushima-ken, Japan;(2) Department of Chemistry, Harvey Mudd College, 91711 Claremont, California, U.S.A.;(3) Pharmaceutical Institute, Tohoku University, Aobayama, 980 Sendai, Miyagi-ken, Japan |
| |
Abstract: | High performance liquid chromatographic separation of a series of mono-, di- and trihydroxylated 5β-cholanic acids which differ
only in position and configuration of hydroxyl groups at positions C-3, C-7 and/or C-12, is reported. The C-24 free acids
were derivatized to four different classes of UV-sensitive esters, i.e.,p-bromophenacyl (BP),m-methoxyphenacyl (MP), 4-nitrophthalimidemethyl (NPM) and 9-anthrylmethyl (AM) esters, and chromatographed on two, variants
of C18 reversed-phase columns (Nova-Pak C18 and Zorbax ODS) with methanol-water systems as mobile phase. Separation efficiency and elution order of some isomeric pairs
were influenced by both the structure of the C-24 ester groups and the nature of the columns used. Excellent chromatographic
properties were found for those derivatives, particularly for the NPM esters. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|