鹰嘴豆膳食纤维对高脂血症大鼠脂代谢的改善作用

本研究观察了鹰嘴豆膳食纤维对高脂血症大鼠血脂及抗氧化水平的影响,为鹰嘴豆保健功效研究提供参考依据。40只雄性SD大鼠高脂饲料喂养建立高脂血症模型,将造模成功的大鼠,随机分为高脂模型组,鹰嘴豆膳食纤维高(30 g/kg高脂饲料)、中(15 g/kg高脂饲料)、低(5 g/kg高脂饲料)3个干预组。高脂模型组继续喂以高脂饲料,干预组分别喂以含不同含量鹰嘴豆膳食纤维的高脂饲料。干预7周后,处理动物,分别测定各组大鼠体重、肝重、附睾周脂重,血清血脂水平及氧化应激水平。结果表明:与高脂模型组相比,鹰嘴豆膳食纤维组体重增量、肝重、附睾周脂重均有下降,其中体重增量以干预第5周高剂量组下降最为明显,降低:29.34%,高中低各剂量组附睾周脂重分别降低:33.81%,17.28%,21.86%,但肝重降低无统计学差异;血脂方面,甘油三酯(TG)、低密度脂蛋白(LDL-C)值高剂量组降低:51.52%,40.45%,总胆固醇(TC)值以中剂量组最优,降低:34.31%,高密度脂蛋白(HDL-C)高剂量组升高42%;在抗氧化能力方面,中剂量组丙二醛(MDA)含量降低40%,高剂量组总超氧化物歧化酶(T-SOD)、谷胱甘肽过氧化物酶(GSH-Px)水平分别升高54%,55%,且T-SOD、GSH-Px升高呈现剂量相关性。可见鹰嘴豆膳食纤维具有降低高脂血症大鼠体质量,调节异常血脂作用,而这种作用可能与其提高高脂血症大鼠抗氧化能力,降低氧化应激损伤有关。

Improvement Effect of Dietary Fiber from Chickpea on Lipid Metabolism in Hyperlipidemic Rats

In this study, the effects of chickpea dietary fiber on blood lipid and antioxidant levels of hyperlipidemic rats were observed to provide a reference for the research of the health benefits of chickpeas. Forty male SD rats were fed with high-fat diet to establish hyperlipidemia model. Then the hyperlipidemia model of rats were randomly divided into hyperlipidemia model group, high chickpea fiber (30 g/kg high-fat diet), middle chickpea fiber (15 g/kg high-fat diet), and low chickpea fiber (5 g/kg high-fat diet) three intervention groups. The hyperlipidemia model group was fed with high-fat diet, and intervention groups were given high-fat diet containing different levels of chickpea dietary fiber. After 7 weeks of intervention, the animals were executed. The body weight, liver weight, epididymal fat weight, serum lipid levels and oxidative stress levels of rats were measured in each group. The results showed that: The weight gain, liver weight and epididymal lipid weight were decreased in chickpea dietary fiber group, compared with the hyperlipidemia model group. After five weeks the body weight was obviously decreased by 29.34%, the epididymal fat weight decreased 33.81%, 17.28% and 21.86% in the high, medium and low dose groups, respectively, but there was no significant difference in liver weight. In terms of blood lipids, triglyceride (TG) and low density lipoprotein (LDL-C) in high-dose group decreased by 51.52% and 40.45%, respectively. The total cholesterol (TC) value was optimal in the middle dose group, which was reduced by 34.31%. High density lipoprotein (HDL-C) in high-dose group increased by 42%. In terms of antioxidant capacity, the malondialdehyde (MDA) content in the middle-dose group was reduced by 40%, the total superoxide dismutase (T-SOD) and glutathione peroxidase (GSH-Px) levels in the high-dose group were increased by 54% and 55%, respectively, the increases of T-SOD and GSH-Px levels were dose-dependent. It can be seen that the dietary fiber of chickpea can reduce body weight and regulate abnormal blood-lipids in hyperlipidemic rats, and this effect may be related to the improvement of antioxidation ability and reduction of oxidative stress injury in hyperlipidemic rats.