ESBL-producing E. coli in Austrian sewage sludge |
| |
| Authors: | Franz Ferdinand Reinthaler Gebhard Feierl Herbert Galler Doris Haas Eva Leitner Franz Mascher Angelika Melkes Josefa Posch Ingrid Winter Gernot Zarfel Egon Marth |
| |
| Affiliation: | 1. International Health Management Associates, Inc., Schaumburg, IL, USA;2. Department of Internal Medicine and Department of Medical Microbiology and Infectious Diseases, School of Medicine, University of Manitoba, Winnipeg, Canada;1. Laboratoire des Microorganismes et Biomolécules actives, Faculté de Sciences de Tunis, Université de Tunis El Manar, 2092 Tunis, Tunisia;2. Laboratory of Epidemiology and Veterinary Microbiology, Pasteur Institute of Tunis, Tunisia;3. Area de Bioquímica y Biología Molecular, Universidad de La Rioja, 26006 Logroño, Spain;4. Institut Supérieur des Sciences Biologiques Appliquées de Tunis, Université de Tunis El Manar, 2092 Tunis, Tunisia;1. Laboratoire des Microorganismes et Biomolécules actives, Faculté de Sciences de Tunis, Université de Tunis El Manar, 2092 Tunis, Tunisia;2. Laboratory of Epidemiology and Veterinary Microbiology, Pasteur Institute of Tunis, Tunisia;3. Area de Bioquímica y Biología Molecular, Universidad de La Rioja, 26006 Logroño, Spain;4. Institut Supérieur des Sciences Biologiques Appliquées de Tunis, Université de Tunis El Manar, 2092 Tunis, Tunisia |
| |
| Abstract: | The aim of this study was to investigate the degree of contamination of sewage sludge with ESBL-producing Escherichia coli strains and the effectiveness of different sewage sludge treatment methods.Monthly sewage sludge samples were collected between January and September 2009 in 5 different sewage treatment plants and tested for the presence of ESBL E. coli. In addition, the number of colony forming units (CFU) of E. coli and coliform bacteria before and after the different sludge treatment methods (aerobic/anaerobic digestion, lime stabilization, and thermal treatment) was investigated.Of the 72 sewage sludge samples investigated, ESBL-positive E. coli were found in 44 (61.1%) sewage sludge samples. The classification of β-lactamase groups was carried out in 15 strains resulting in the detection of 2 different groups (CTX-M and TEM) of bla genes. All 15 of them had a CTX-M gene and 4 of these strains furthermore carried a TEM gene.With regard to the CFU of E. coli and coliform bacteria, thermal treatment and lime stabilization following dehydration sufficiently reduced pathogen concentrations. The plants using merely stabilization and dehydration showed an increase of E. coli and coliform bacteria and thus also an increase in ESBL-producing E. coli. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
