蜂胶醇提物对人肝癌细胞Hep G2增殖及凋亡的影响

为探讨蜂胶醇提物对人肝癌细胞Hep G2 增殖和凋亡的影响，采用MTT 法检测蜂胶醇提物对Hep G2 细胞增殖的抑制作用，用荧光倒置显微镜和流式细胞仪分析蜂胶醇提物对Hep G2 细胞凋亡的影响。结果表明：12.5～200μg/mL 质量浓度的蜂胶醇提物作用24、48h 后，均能不同程度地抑制Hep G2 细胞的增殖，呈明显的时间、剂量依赖关系，半数抑制质量浓度(IC50)分别是115、78μg/mL。作用8h 后，Hep G2 细胞出现不同程度的凋亡，凋亡率由6.36% 上升到21.9%，呈现出剂量依赖关系。故蜂胶醇提物可显著抑制人肝癌细胞Hep G2 的生长，诱导其凋亡。

Effect of Ethanol Extract of Propolis on Proliferation and Apoptosis of Human Liver Carinoma Hep G2 Cells

In order to investigate the effect of propolis on cell proliferation and apoptosis, the ethanol extract of propolis was used to treat human liver carcinoma Hep G2 cells. The inhibitory rate of ethanol extract from propolis on the proliferation of human liver carcinoma Hep G2 cells was determined by MTT assay and the apoptosis of human liver carcinoma Hep G2 cells was examined by using inverted fluorescence microscope and flow cytometry analysis. Results indicated that 12.5－200 μg/mL ethanol extract of propolis exhibited an obvious inhibition effect on the proliferation of Hep G2 cells after 24 h and 48 h treatment in a time- and dose-dependent manner. The apoptosis of Hep G2 cells was obviously observed after treatment and apoptosis rate was in the range of 6.36% to 21.9% in a dose-dependent manner. These results confirm that propolis can significantly inhibit the proliferation and induce apoptosis of Hep G2 cells.