金柚皮总黄酮的微波辅助提取工艺研究

采用微波辐射预处理金柚皮，以乙醇作提取剂从金柚皮中提取黄酮类物质，探讨了影响金柚皮总黄酮提取的微波辐射预处理条件(辐射功率和辐射时间)、乙醇浓度、浸提时间、浸提温度、料液比因素。通过正交试验确定了最佳工艺条件。结果表明，在微波辐射功率495W、辐射时间3min 预处理金柚皮后，用80% 乙醇作为浸提剂，按料液比1:8，在温度70℃下浸提40min 可取得最佳提取效果。此条件下总黄酮提取率达0.99%。

Microwave-assisted Extraction of Total Flavonoids from Fruit Peels of Shatian Pomelo (Citrus grandis var. shatinyu Hort)

This study aimed to optimize extraction technology of total flavonoids from microwave-pretreated fruit peel slices of Shatian pomelo with ethanol. Two parameters of microwave pretreatment, including radiation power and duration, were optimized by single factor test, and then four parameters of extraction, including ethanol concentration, extraction temperature and duration, and solid/liquid ratio, were optimized by orthogonal test design based on single factor test. Results showed that under optimized conditions with microwave-pretreated fruit peel slices of Shatian pomelo for 3 min at 495 W as the material and 80% ethanol as the extraction solvent, as well as the ratio between them of 1:8 (g/ml), extraction for 40 min at 70 ℃], the best extraction efficiency of total flavonoids was achieved. Triple validation experiment on the optimal extraction technology obtained showed that average extraction yield of total flavonoids was 0.99%.