猪肚菇子实体多糖的分离纯化及抗氧化活性测定

目的：研究多猪肚菇子实体多糖的抗氧化活性。方法：用热水浸提新鲜猪肚菇子实体，乙醇沉淀多糖，Sevag 法去除多糖中的蛋白，DEAE-52 纤维素柱层析、Sephadex S-200 凝胶柱层析进一步纯化，然后进行抗氧化活性测定。结果：DEAE 柱层析纯化后得到两种多糖组分A 和B，Sephadex S-200 凝胶柱层析证实A 为单一组分，组分A 的抗氧化活性测定结果显示其具有抗氧化活性，且抗氧化活性随其质量浓度的升高而升高。结论：分离纯化后的猪肚菇多糖具有抗氧化活性，且活性呈现剂量效应，随质量浓度升高而升高。

Isolation,Purification and Antioxidant Activity of Polysaccharides from the Fruit Body of Panus giganteus (Berk.) Corner

The polysaccharides in the fruit body of Panus giganteus (Berk.) Corner were extracted with hot water, and precipitated with ethanol. The crude polysaccharides were then deproteinized by Sevage method, followed by DEAE 52 column chromatographic purification/fractionization. As a result, 2 fractions were obtained. Finally, fraction A, the first elution peak on DEAE 52 column was further purified by Sephadex S-200 column chromatography, and only one peak was observed in the elution profile, indicating this faction contains only a single component. It was found that fraction A could inhibit lipid peroxidation in mouse liver homogenate in vitro in a concentration-dependent way.