RP-HPLC测定金针菇中芦丁、槲皮素和丁香酸的含量

采用高效液相色谱法测定金针菇中的多酚类成分，使用Agilent ZORBAX SB-C18 分析柱(4.6mm × 250mm，5μm)，流动相为乙腈(A)-0.4%乙酸(B)，其中0～40min 为5% A-95% B，40～45min 为25% A-75% B，45～50min为35% A-65% B，之后运行5min，流速1.0ml/min，VWD 检测器，检测波长280nm，柱温30℃。本研究在鲜金针菇中检出芦丁和槲皮素、在干金针菇中检出槲皮素和丁香酸。

RP-HPLC Determination of Rutin,Quercetin and Syringic Acid in Flammulina velutipe Fruitbody

A HPLC method was established to determine polyphenolic compounds in Flammulina velutipe fruitbody. The chromatographic separation was achieved on an Agilent Zorbax SB-C18 (4.6 mm × 250 mm, 5 μm) analytical column using a mobile phase consisting of a mixture of A: acetonitrile and B: 0.4% acetic acid (0–40 min: 5% A and 95% B; 40–45 min: 25% A and 75% B; 45–50 min: 35% A and 65% B) at a flow rate of 1.0 ml/min and 30 ℃ column temperature , and the analytes were detected using a VWD detector at 280 nm wavelength. Rutin and quercetin both were found in fresh Flammulina velutipe fruitbody, while quercetin, together with syringic acid was detected in dried samples.