| 高效液相色谱法测定虾壳中的虾青素 |
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| 引用本文： | 姜 淼,杨贤庆,李来好,岑剑伟,郝淑贤,魏 涯,陈胜军,戚 勃.高效液相色谱法测定虾壳中的虾青素[J].食品科学,2010,31(20):371-375. |
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| 作者姓名： | 姜 淼 杨贤庆 李来好 岑剑伟 郝淑贤 魏 涯 陈胜军 戚 勃 |
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| 作者单位： | 1.中国水产科学研究院南海水产研究所
2.广东海洋大学食品科技学院 |
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| 基金项目： | “十一五”国家科技支撑计划项目(2008BAD94B02)；广东省海洋渔业科技推广专项(A2009001-011(b)；
A200901B02；A200901I03)；农业部中央级公益性科研院所基本科研项目(2010YD08；2010TS09) |
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| 摘 要： | 建立高效液相色谱法(HPLC)测定虾壳中虾青素的方法。通过正交试验考察虾壳中虾青素的最佳提取条件。结果表明，以乙酸乙酯为提取剂，超声波辅助处理30min，后振荡15min，重复两次，虾青素可被充分提取。该方法虾青素质量浓度在0.2～10μg/mL 内具有良好的线性关系(R2=0.9999)，回收率在80.41%～109.21% 之间，相对标准偏差在4.65%～7.45% 之间，虾青素定量限为0.075mg/kg。该法分析快速准确、灵敏度高、重现性好，回收率高。
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| 关 键 词： | 虾壳 虾青素 正交法 高效液相色谱法 |
| 收稿时间： | 2010-06-30 |
Determination of Astaxanthin in Shrimp Shells by High Performance Liquid Chromatography |
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| JIANG Miao,YANG Xian-qing,LI Lai-hao,CEN Jian-wei,HAO Shu-xian.Determination of Astaxanthin in Shrimp Shells by High Performance Liquid Chromatography[J].Food Science,2010,31(20):371-375. |
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| Authors: | JIANG Miao YANG Xian-qing LI Lai-hao CEN Jian-wei HAO Shu-xian |
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| Affiliation: | 1. South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China；
2. College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China |
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| Abstract: | A method for determining astaxanthin in shrimp shells was established using high performance liquid chromatography (HPLC). The optimal conditions for sample preparation were explored by orthogonal array design to be extraction with ethyl acetate under the assistance of ultrasound treatment for 30 min followed by oscillation for 15 min and the procedure was repeated twice for full extraction of astaxanthin. A good linear relationship determined by HPLC method was observed when astaxanthin concentration was in the range of 0.2 to 10μg/mL (R2 = 0.9999). The recovery rates for astaxanthin in a spiked sample ranged between 80.41% and 109.21% with relative standard deviations (RSD) between 4.65% and 7.45%. The limit of detection was 0.075 mg/kg. This method is characteristic of rapidity, accuracy, high sensitivity and recovery rate and good reproducibility. |
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| Keywords: | shrimp shell astaxanthin orthogonal array design HPLC |
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