| 纳米低温保护剂提高卵母细胞玻璃化保存效果的机理初探 |
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| 引用本文： | 李维杰,周新丽,吕福扣,刘宝林.纳米低温保护剂提高卵母细胞玻璃化保存效果的机理初探[J].制冷学报,2014(1):114-118. |
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| 作者姓名： | 李维杰 周新丽 吕福扣 刘宝林 |
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| 作者单位： | 上海理工大学生物热科学研究所; |
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| 基金项目： | 国家自然科学基金(50906057,51076108)资助项目;上海教委科研创新项目(14YZ098)资助~~ |
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| 摘 要： | 纳米颗粒添加到低温保护剂中可能是未来低温保存的重要手段。采用添加了羟基磷灰石(HA)纳米颗粒的低温保护剂冷冻卵母细胞,使用低温显微镜观察了结晶、再结晶和融化时冰晶的形态和细胞的变化,同时记录各现象发生的温度。发现添加0.05%HA组在升温过程中再结晶不明显,且再结晶危险区较小,对细胞无损伤;而未添加HA组细胞在复温过程中受伤几率很高。结果表明纳米颗粒减少了复温过程中的再结晶可能是纳米低温保护剂提高细胞存活率的主要机理之一。
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| 关 键 词： | 低温生物 重结晶 低温显微镜 纳米低温保护剂 |
Preliminary Study on the Mechanism of Promoting the Survival Rate of Vitrified Oocytes with Nano-cryoprotectants |
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| Affiliation: | Institute of Biothermal Technology, University of Shanghai for Science and Technology;Institute of Biothermal Technology, University of Shanghai for Science and Technology;Institute of Biothermal Technology, University of Shanghai for Science and Technology;Institute of Biothermal Technology, University of Shanghai for Science and Technology |
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| Abstract: | Adding nanoparticles into cryoprotectant may be an important means of cryopreservation in future. The oocytes were cryopreserved using cryoprotectant containing hydroxyapatite (HA), and the morphology of crystal and oocytes in the process of crystallization, recrystallization and thawing were observed using cryomicroscope system. In the mean time, the temperatures were recorded when every phase change occurred. It is found that cryoprotectant with 0.05%HA recrystallized implicitly with narrow danger zone during rewarming and hence little harm was done to oocytes. In contrast, the group without any HA nanoparticles tended to get harmed. In conclusion, the mechanism that nano-cryoprotectant promoted the survival rate of oocytes was that nanoparticles hindered the recrystallization of cryoprotectant in the process of rewarming. |
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