| 响应面法优化莜麦蛋白微波辅助碱法提取工艺 |
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| 引用本文： | 宁芯,班薇薇,王国泽,莫紫梅,汪磊.响应面法优化莜麦蛋白微波辅助碱法提取工艺[J].食品工业,2020(3):99-102. |
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| 作者姓名： | 宁芯 班薇薇 王国泽 莫紫梅 汪磊 |
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| 作者单位： | 玉林师范学院化学与食品科学学院;广西农产资源化学与生物技术重点实验室;内蒙古科技大学生命科学与技术学院;广西-东盟食品药品安全检验检测中心 |
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| 基金项目： | 内蒙古科技大学创新基金(No.2014QNGG06);玉林师范大学大学生创新创业项目(201810606011);玉林师范学院高层次人才科研启动基金(G2018011);广西高校中青年教师基础能力提升项目(2018KY0468);广西重点研发计划(2018AB45026);国家自然科学基金(31960478)。 |
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| 摘 要： | 为提高莜麦蛋白的提取率,采用微波辅助碱法提取莜麦蛋白。在单因素试验的基础上,利用Box-Behnken中心组合试验和响应面分析法优化莜麦蛋白微波辅助碱法提取工艺条件。结果表明:微波辅助碱法提取莜麦蛋白的最佳工艺参数为:微波功率380 W、pH 9.45、料液比1︰23(g/mL)、提取时间7.75 min。该条件下莜麦蛋白的提取率为79.24%。与常规碱法提取莜麦蛋白相比,微波辅助提取法能大幅减少提取时间,显著提高莜麦蛋白的提取率。
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| 关 键 词： | 莜麦蛋白 碱法提取 微波辅助 响应面法 |
Optimization of Microwave Assisted Extraction of Naked Oat Protein by Response Surface Methodology |
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| NING Xin,BAN Weiwei,WANG Guoze,MO Zimei,WANG Lei.Optimization of Microwave Assisted Extraction of Naked Oat Protein by Response Surface Methodology[J].The Food Industry,2020(3):99-102. |
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| Authors: | NING Xin BAN Weiwei WANG Guoze MO Zimei WANG Lei |
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| Affiliation: | (College of Chemistry and Food Science,Yulin Normal University,Yulin 537000;Guangxi Key Laboratory of Agricultural Resources Chemistry and Biotechnology,Yulin 537000;School of Life Science and Technology,Inner Mongolia University of Science and Technology,Baotou 014010;Guangxi-ASEAN Center for Food and Drug Safety Control,Nanning 530001) |
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| Abstract: | Microwave-assisted alkali extraction method was used to enhance the extraction efficiency of naked oat protein.On the basis of single-factor experiments,Box-Behnken center-united experiment design coupled with response surface methodology was used to optimize the extraction technology of naked oat protein.The results showed that the optimal microwave assisted extraction technology of naked oat protein was microwave power 380 W,pH 9.45,ratio of material and liquid 1︰23(g/mL),and microwave time 7.75 min.Under this condition,the extraction rate could amount to 79.24%.Compared with the alkaline collection,microwave assisted extraction technology not only reduced extraction time,but also improved extraction rate significantly. |
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| Keywords: | naked oat protein alkali extraction microwave assisted response surface methodology |
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