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| 壳聚糖与羧甲基壳聚糖蛋白质印迹聚合物的制备及吸附性能 | |
| 引用本文： | 王艺峰,王立莹,刘洲,曾珊珊,徐敏. 壳聚糖与羧甲基壳聚糖蛋白质印迹聚合物的制备及吸附性能[J]. 高分子材料科学与工程, 2011, 0(9): 126-129 |
| 作者姓名： | 王艺峰 王立莹 刘洲 曾珊珊 徐敏 |
| 作者单位： | 武汉理工大学材料科学与工程学院 |
| 基金项目： | 国家自然科学基金面上项目(50703030);“国家大学生创新性实验计划”资助 |
| 摘 要： | 首先制备了壳聚糖的衍生物——羧甲基壳聚糖,再以壳聚糖与羧甲基壳聚糖的共混物为功能单体,牛血清白蛋白(BSA)为模板蛋白质,制备了一种壳聚糖与羧甲基壳聚糖共混物的蛋白质印迹聚合物。模板蛋白质吸附测试结果表明,该蛋白质印迹聚合物对BSA的吸附量是非印迹聚合物的30.8倍;对不同蛋白质的吸附测试结果表明,相比于其它对比蛋白质,该蛋白质印迹聚合物具有良好的选择性吸附模板蛋白质BSA的效果;并且该蛋白质印迹聚合物具有良好的可重复使用性能。 |
| 关 键 词： | 壳聚糖羧甲基壳聚糖 分子印迹 蛋白质印迹聚合物 蛋白质选择性吸附与分离 |
Protein Imprinted Polymer of Chitosan and Carboxymethylated Chitosan |
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| Yifeng Wang,Liyin Wang,Zhou Liu,Shanshan Zeng,Min Xu. Protein Imprinted Polymer of Chitosan and Carboxymethylated Chitosan[J]. Polymer Materials Science & Engineering, 2011, 0(9): 126-129 | |
| Authors: | Yifeng Wang Liyin Wang Zhou Liu Shanshan Zeng Min Xu |
| Affiliation: | (School of Materials Science and Engineering,Wuhan University of Technology,Wuhan 430070,China) |
| Abstract: | In this work,a carboxymethylated chitosan(CMCS) has been synthesized.Then,a protein imprinted polymer(CS/CMCS-MIP) was prepared by using chitosan and carboxymethylated chitosan compound as functional matrix,bovine serum albumin(BSA) as template molecule,and epoxy chloropropane as the crosslinker.The template protein adsorption experiments show that BSA adsorption capacity of the protein imprinted polymer is 30.8 times that of the non-imprinted polymer.The adsorption experiments to different proteins indicate that the protein imprinted polymer has good selective adsorption capacity towards the template protein BSA.In addition,the protein re-adsorption tests show that the protein imprinted polymer has good regeneration and reproducibility property. |
| Keywords: | carboxymethylated chitosan molecular imprinting protein imprinted polymer protein selective adsorption and separation |
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