液芯波导对溶液拉曼光谱探测灵敏度的放大

采用折射率比石英(1．46)低的Teflon-AF(n=1.29)液芯波导系统。研究了乙醇和四氯化碳溶液的拉曼光谱，通过利用液芯波导的内全反射特性。延长激发光对波导管内的溶液试样的总的有效激发光程。并有效增强对光谱信号的收集，成功地采集到四氯化碳和乙醇的拉曼光谱。并与采用常规90度几何散射结构获得的拉曼光谱进行了对比，表明液芯波导系统可以使拉曼光谱信号强度增强200倍以上，并大大提高探测灵敏度和信噪比。展示了液芯波导拉曼光谱应用于化合物溶液的微量分析、生物医药成分的定量检测、临床医学诊断中对病理样本的微量分析和定量检测等领域的可行性，为进一步采用液芯波导拉曼光谱进行定量标定的研究工作和实际应用提供了实验依据。这项技术也适合于对其它包含多种成分的复杂的生物体系和化学药品进行定量分析，具有巨大的应用前景和实际价值。

Raman sensitivity enhancement for liquid samples using a liquid waveguide capillary cell

We use a long Teflon-AF2400 liquid waveguide capillary cell(LWCC)to study Raman spectroscopy of alcohol and tetrachloride.LWCC can provide the enhancement of signal intensities and detection sensitivity in liquid sample Raman spectroscopy measurements,in which the optical path length through the liquid sample is much longer than it would normally be,and Raman scattering light emitted by the sample can also be trapped by LWCC.In our work,we have demonstrated the use of a Liquid waveguide cell to measure Raman spectrum of alcohol and tetrachloride.We also compare Raman Spectra of alcohol and tetrachloride collected in LWCC to those collected in a traditional 90 0 scattering geometry,and obtain a detection sensitivity enhancement factor of more than 200.It have shown that LWCC can provide much higher Raman signal intensities while simultaneously significantly reducing sample volume requirements.This result can be used to evaluate the potential of Raman spectroscopy using LWCC to perform microanalysis and measurements of liquid samples.This technique is capable of measuring the concentration of other Raman-active liquid samples and complicated biology system.