| 微小毛霉发酵制取凝乳酶的培养基优化研究 |
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| 引用本文： | 张丽红,王昕,朴姍善,郑丽,杨贞耐. 微小毛霉发酵制取凝乳酶的培养基优化研究[J]. 食品工业科技, 2011, 0(9): 214-216,219 |
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| 作者姓名： | 张丽红 王昕 朴姍善 郑丽 杨贞耐 |
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| 作者单位： | 1. 吉林大学生物与农业工程学院,吉林长春130024／中国农业科技东北创新中心农产品加工研究中心,吉林长春130033
2. 吉林大学生物与农业工程学院,吉林长春,130024 |
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| 基金项目： | 863现代农业探索导向项目(2006AA10Z306); 农业部现代奶牛产业技术体系乳制品加工专项、科学前沿与交叉学科创新项目(200903276) |
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| 摘 要： | 利用响应面分析法优化微小毛霉的发酵培养基,提高微小毛霉凝乳酶的凝乳活力。在单因素实验的基础上,用Design-Expert软件对实验数据进行多元回归分析,建立了3种因素与凝乳酶活力之间的函数关系,得出在基础发酵培养基中加入的氯化钙、乳清粉和葡萄糖的最佳浓度分别为0.58%、0.64%和1.13%,此时,微小毛霉凝乳酶的凝乳活力的理论值为1150.81SU/mL,验证平均值为1109.7SU/mL,与预测值基本一致。
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| 关 键 词： | 凝乳酶 微小毛霉 发酵培养基 优化 |
Optimization of fermentation medium to producing chymosin by Mucor pusillus |
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| ZHANG Li-hong,,WANG Xin,PIAO Shan-shan,ZHENG Li,YANG Zhen-nai. Optimization of fermentation medium to producing chymosin by Mucor pusillus[J]. Science and Technology of Food Industry, 2011, 0(9): 214-216,219 |
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| Authors: | ZHANG Li-hong WANG Xin PIAO Shan-shan ZHENG Li YANG Zhen-nai |
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| Affiliation: | ZHANG Li-hong1,2,WANG Xin1,PIAO Shan-shan1,ZHENG Li1,YANG Zhen-nai1,*(1. School of Biological and Agricultural Engineering Jilin University,Changchun 130024,China,2. Northeast Agricultural Research Center of China,Jilin Academy of Agricultural Sciences,Changchun 130033,China) |
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| Abstract: | Optimization of the medium for fermentation by Mucor pusillus to produce chymosin was carried out by response surface analysis to increase the activity of milk-clotting enzyme(MCE).Based on the single factor test,the polynomial regression analysis was performed on the experimental data by using Design-Expert software and the functional relationship between the three primary components and the MCE activity were built.The optimal values of the factors were obtained:CaCl2 was 0.58%,whey powder was 0.64%,glucos... |
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| Keywords: | chymosin Mucor pusillus fermentation medium optimization |
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