Construction of proteinase A deficient transformant of industrial brewing yeast |
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| Authors: | Zhao-Yue Wang Guo-Qing He Hui Ruan Zhong-Shan Liu Lu-Fang Yang Bo-Run Zhang |
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| Affiliation: | (1) Department of Biosystem Engineering and Food Science, Zhejiang University, Hangzhou, P.R. China;(2) China Lion Brewery Group, Wenzhou, P.R. China;(3) The Lab of Breeding and Molecular Genetics of Yeast, Institute of Microbiology, Chinese Academy of Sciences, Beijing, P.R. China |
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| Abstract: | Yeast proteinase A is detrimental to beer foam. The proteinase A deficient transformant of industrial brewing yeast, WZ65/a,
was constructed using PCR-mediated gene disruption, and the transformant was verified to be genetically stable. The PCR analysis
showed that PEP4 gene coding for proteinase A in the WZ65/a was disrupted. No matter in the yeast cells or in the fermenting liquor of WZ65/a,
proteinase A activity could not be detected. Analysis of the main charicteristics indexes of beer also showed that proteinase
A activity and foam performance in the beer brewed with WZ65/a were better than that of the host strain, WZ65. |
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| Keywords: | PEP4 Proteinase A deficient transformant PCR-mediated gene disruption Foam stability |
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