Determination of lutein and zeaxanthin and antioxidant capacity of supercritical carbon dioxide extract from daylily (Hemerocallis disticha) |
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| Authors: | Yu-Wen Hsu Chia-Fang Tsai Wen-Kang Chen Yung-Chyuan Ho Fung-Jou Lu |
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| Affiliation: | 1. School of Optometry, Chung Shan Medical University, No. 110, Sec. 1, Jianguo N. Rd., Taichung City 402, Taiwan;2. Department of Biotechnology, Trans world University, No. 1221, Zhennan Rd., Douliu City, Yunlin Country 640, Taiwn;3. Department of Applied Cosmetology, National Tainan Institute of Nursing, No. 78, Sec. 2, Minzu Rd., Tainan City, Taiwan;4. Institute of Medicine, College of Medicine, Chung Shan Medical University, No. 110, Sec. 1, Jianguo N. Rd., Taichung City 402, Taiwan;5. Department of Nutrition, Chung Shan Medical University Hospital, No. 110, Sec. 1, Jianguo N. Rd., Taichung City 402, Taiwan |
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| Abstract: | Lutein and zeaxanthin were extracted from daylily (Hemerocallis disticha) flowers using supercritical fluid extraction-carbon dioxide (SFE-CO2) at a temperature range of 50–95 °C and pressure range of 300–600 bar. The extracts were analysed by HPLC with a C30 column and an isocratic solvent system: methanol/methyl-tert-butyl ether = 86/14 (v/v). Moreover, the antioxidant capacities of the extracts were evaluated by a 2,2-diphenyl-2-picrylhydrazyl radical scavenging assay and a chemiluminescence assay to measure the scavenging activity of hydrogen peroxide, superoxide anion and hydroxyl radical. The optimal lutein and zeaxanthin extraction could be achieved at 80 °C and 600 bar, and the extraction pressure was the most important parameter for SFE-CO2. In addition, the extracts had significantly higher antioxidant activities in all antioxidant assays. |
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| Keywords: | Daylily flower Lutein Zeaxanthin Supercritical fluid extraction-carbon dioxide HPLC Antioxidant capacity |
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