| 脊髓灰质炎病毒与痘苗病毒重组体的构建及表达 |
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| 引用本文： | 戴长柏,李奇涵,袁天喜,胡凝珠,苏晔,郭仁,候云德.脊髓灰质炎病毒与痘苗病毒重组体的构建及表达[J].粉末涂料与涂装,1991(4). |
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| 作者姓名： | 戴长柏 李奇涵 袁天喜 胡凝珠 苏晔 郭仁 候云德 |
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| 作者单位： | 中国医学科学院医学生物学研究所 昆明650107(戴长柏，李奇涵，袁天喜，胡凝珠，苏晔，郭仁)，中国预防医学科学院病毒学研究所 北京(候云德) |
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| 摘 要： | 含脊髓灰质炎病毒1型(简称 PV1)全基因7.4Kb cDNA 和编码 PV1外壳蛋白 VP12.5Kb 的 cDNA 片段的两株重组痘苗病毒(RV-1和 RV-2)表达了 PV1抗原。经荧光标记的抗 PV1型特异性单克隆抗体检测,感染了 RV-1和 RV-2的 Herp-2细胞呈阳性荧光反应。免疫印迹实验证实两株重组痘苗病毒分别表达 PV1抗原三条和两条特异性蛋白带。免疫家兔后能诱导产生抗 PV1中和抗体。本文对重组病毒表达 PV1抗原的效率进行了初步讨论。
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| 关 键 词： | 脊髓灰质炎病毒 重组痘苗病毒基因表达 |
STUDY OF CONSTRUCTION AND EXPRESSION IN POLIO-VACCINIA VIRUS RECOMBINANT VIRUS |
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| Dai Changbai Institute of Medical Biology,Chinese Academy of Medical Sciences,Kunming.STUDY OF CONSTRUCTION AND EXPRESSION IN POLIO-VACCINIA VIRUS RECOMBINANT VIRUS[J].Chinese Journal of Biologicals,1991(4). |
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| Authors: | Dai Changbai Institute of Medical Biology Chinese Academy of Medical Sciences Kunming |
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| Abstract: | Two recombinant vaccinia virus(RV-1 and RV-2)strains containing a full genome of polio virus(PV)type Ⅰ and encoding structural protein Vpl 2.5 Kd cDNA of PV have successfully expressed the antigenic protein of PV type Ⅰ.The antigen was located in the infected Hep-2 cell with an appearence specific granular particles by IF assay.The expressed PV proteins showed as four bands in RV-1 and two bands in RV-2 on Western Blot.They reacted well with anti-PV McAb.The animals immunlized with the two recombinant virus strain could produce neutralization antibodies against PV type Ⅰ.This paper also presented the preliminary results of expression level of PV type Ⅰ anti- gen of recombinant virus |
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| Keywords: | Poliovirus Recombinant VV Gene expression |
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