| 细脚拟青霉产胞外抗氧化活性成分培养基的优化 |
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| 引用本文： | 葛飞,桂琳,汤斌,陶玉贵,黄寅,李婉珍.细脚拟青霉产胞外抗氧化活性成分培养基的优化[J].安徽机电学院学报,2009(1). |
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| 作者姓名： | 葛飞 桂琳 汤斌 陶玉贵 黄寅 李婉珍 |
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| 作者单位： | 安徽工程科技学院微生物发酵安徽省工程技术研究中心 皖南医学院微生物和免疫学教研室 |
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| 基金项目： | 安徽工程科技学院引进人才科研启动基金资助项目(2007yq005);; 安徽省高校自然科学基金资助项目(kj2009b071) |
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| 摘 要： | 采用液体摇瓶培养的方法,以DPPH自由基清除率为指标,对一株细脚拟青霉产胞外抗氧化活性成分培养基进行了优化,确定了其产抗氧化活性物质的最佳培养基.结果表明最佳培养基为:碳源为蔗糖和麦芽糖组成的复合碳源;氮源为酵母浸出粉;微量元素及生长因子为KH2PO4、柠檬酸铵和VB;正交实验结果表明最佳培养基配方为蔗糖2%、麦芽糖1%、酵母浸出粉1.5%、KH2PO40.5%、柠檬酸铵0.04%、VB0.03%.培养基优化后,该菌株发酵液中代谢产物的抗氧化活性可达73.47%.
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| 关 键 词： | 细脚拟青霉 自由基清除率 培养基配方 液体培养 |
Optimization of medium of producing antioxidant from fermentation broth of Paecilomyces tenuipes |
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| GE Fei,; GUI Lin,; TANG Bin,; TAO Yu-gui; HUANG Yin,; LI Wan-zhen.Optimization of medium of producing antioxidant from fermentation broth of Paecilomyces tenuipes[J].Journal of Anhui Institute of Mechanical and Electrical Engineering,2009(1). |
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| Authors: | GE Fei ; GUI Lin ; TANG Bin ; TAO Yu-gui; HUANG Yin ; LI Wan-zhen |
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| Affiliation: | 1(1.Anhui Province Engineering Technology Research Center of Microbial Fermentation; Anhui University of Science and Technology; Wuhu 241000; China; 2.Dept of Micr.& immun.; Wannan Medical College; China); |
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| Abstract: | The optimum medium of producing antioxidant from fermentation broth of Paecilomyces tenuipes in shaking flask was investigated with the method of DPPH.The results show that the formula of appropriated culture medium is as follows: sucrose 2%,maltose 1%,yeast powder 1.5%,KH2PO4 0.5%,ammonium citrate 0.04% and VB 0.03%.The free radical scavenging rate of fermentation broth reaches 73.47% after medium optimization. |
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| Keywords: | paecilomyces tenuipes free radical scavenging rate media components liquid culture |
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