黄芩多糖大孔树脂纯化工艺优化及其抑菌活性

为优化大孔树脂纯化黄芩粗多糖的工艺,比较不同类型树脂的静态吸附与解吸性能,确定适宜树脂型号,研究其吸附机理.通过动态吸附与洗脱试验确定最佳纯化工艺,同时利用滤纸片法考察黄芩多糖对大肠杆菌、枯草杆菌和金黄色葡萄球菌的抑菌效果.结果表明,AB-8型大孔树脂对黄芩多糖的吸附过程符合Langmuir模型特征,最佳纯化工艺条件为...

Optimization Purification Process of Polysaccharides from Scutellaria baicalensis Georgi by Macroporous Resins and Its Antibacterial Activity

To optimize the purification conditions of polysaccharides extract from Scutellaria baicalensis Georgi by macroporous resin, the suitable macroporous resin was selected through performance comparison of static adsorption and elution of macroporous resins of different types, and the optimum conditions were confirmed by dynamic adsorption and elution tests. Meanwhile, the resistant abilities against Escherichia coli, Staphylococcus aureus, and Bacillus subtilis were detected by filtering paper method. The results showed that the adsorption curves that AB-8 macroporous resin adsorbed polysaccharides of Scutellaria baicalensis Georgi accorded with the Langmuir mode feature. The optimum purification conditions were as follows: 60 mL polysaccharides extract solution of concentration 3 mg/mL (pH 5.0) was made and adsorbed by AB-8 macroporous resin with loading flow rate 2.0 mL/min, and then eluted by 75% ethanol with elution flow rate 1.0 mL/min. Under these conditions, the total polysaccharides content increased from 26.25% to 77.12%. The polysaccharides of Scutellaria baicalensis Georgi had inhibitory effects varying degrees on the four tested bacteria and the strongest inhibitory activity was resistant against Escherichia coli. The minimum inhibitory concentrations of Escherichia coli, Staphylococcus aureus, and Bacillus subtilis were 0.625, 0.625 and 1.25 mg/mL, respectively. This process had better purified efficiency, and polysaccharides after purification had better antibacterial activities, therefore it provides a reference for corresponding resource development.