高效液相色谱-串联质谱同时检测黑巧克力中的五种大麻酚

基于巧克力和大麻酚类物质的物理化学性质，本文通过对前处理方法和检测方法的优化，建立了一种巧克力中五种大麻酚的高效液相色谱-串联质谱检测方法。并通过精密度、重复性以及加标回收等实验对方法的有效性进行了验证。结果表明，五种大麻酚在0.05~5.0 μg/mL浓度范围内有良好的线性关系，线性相关系数R2≥0.997，定量限（LOQ，S/N=10）为0.15 mg/kg；日内和日间精密度的相对标准偏差RSDs值<5%，说明该方法具有良好的精密度和稳定性；在三个加标水平（1.25、2.50、5.00 mg/kg）下的回收率为91.8%~109.0%，RSDs<5%，说明该方法具有较高的准确性。本方法具有简单、快速、准确等优点，可以为建立巧克力中大麻酚类物质的检测方法标准奠定实验基础，同时为加强国际大麻酚添加食品进出口监管提供技术储备。

Simultaneous Determination of Five Cannabinoids in Dark Chocolate with High Performance Liquid Chromatography Tandem Mass Spectrometry

Based on the physicochemical properties of chocolate and cannabinoids, a method for the determination of five cannabinoids in dark chocolate by high performance liquid chromatography tandem mass spectrometry was developed by optimizing the pretreatment and detection methods. The validity of the method was verified by precision, repeatability and recovery experiments. Results showed that the linear range of five cannabinoids was 0.05~5.0 μg/mL with the linear correlation coefficient R2≥0.997, the limit of quantitation (LOQ, S/N=10) was 0.15 mg/kg. The RSDs of intraday precision and interday precision were below 5%, which indicated that the method had good precision and stability. The recoveries at three levels (1.25, 2.50, 5.00 mg/kg) were from 91.8% to 109.0%, with RSDs<5%, indicating that the method had high accuracy. The established method is simple, rapid and accurate, and it can lay an experimental foundation for the establishment of the standard method for the determination of cannabinoids in chocolate, and provide technical reserves for strengthening the international supervision of import and export of cannabinoids added food.