气相色谱-氢火焰离子化检测器法测定白酒中50种风味物质

为满足企业对白酒中多种风味物质进行快速准确地定量分析的需求，以叔戊醇、乙酸正戊酯、2-乙基丁酸为内标物，建立气相色谱-氢火焰离子化检测器（GC-FID）检测白酒中50种挥发性风味物质的方法。结果表明，色谱条件为CP-WAX57CB色谱柱（50 m×0.25 mm×0.20 μm），载气为高纯氮气（N2），流速1.0 mL/min。进样量1 μL，分流比20∶1，进样口温度250 ℃；检测器温度250 ℃；标准品比对进行定性，内标法定量。50种风味物质在各自质量浓度范围内线性关系良好，相关系数（R2）均＞0.995。精密度试验结果的相对标准偏差（RSD）均＜5%，检出限在0.34～25.62 mg/L之间，加标回收率在90.06%～106.30%之间。该方法简单，精密度及准确度均满足白酒中风味物质检测的要求，可用于白酒中50种风味物质含量的检测分析。

Determination of 50 flavor substances in Baijiu by gas chromatography-flame ionization detector

In order to meet the needs of rapid and accurate quantitative analysis of various flavor substances of Baijiu (Chinese liquor) in enterprise, using tert- amyl alcohol, n-amyl acetate and 2-ethylbutyric acid as internal standard compounds, a method for the determination of flavor substances in Baijiu by gas chromatography-flame ionization detector (GC-FID) was developed. The results showed that the chromatographic condition was CP-WAX57CB analysis column (50 m×0.25 mm×0.20 μm), high purity nitrogen as carrier gas, flow rate 1.0 ml/min, sampling amount 1 μl, shunt injection with ratio 20∶1, sample inlet temperature 250 ℃, detector temperature 250 ℃, and standard comparison for qualitative, internal standard method for quantification. All the 50 flavor substances had good linear relationship in the linear range, and the correlation coefficient R2 was above 0.995. The relative standard deviation (RSD) of precision tests results was less than 5%, the detection limit was 0.34-25.62 mg/L, and the adding standard recovery rate was in the range of 90.06%-106.30%. The method was simple, and its precision and accuracy were all in line with the requirements of flavor substance in Baijiu detection, which could be used for simultaneous determination and analysis of 50 flavor substances in Baijiu.