|
|||||
|
|
| 不同炮制方法的杜梨叶中总黄酮含量测定 | |
| 引用本文： | 张岭苓,李楠,徐凌川,秦国培,殷法杰,蒋海强. 不同炮制方法的杜梨叶中总黄酮含量测定[J]. 食品科技, 2011, 0(10): 269-271 |
| 作者姓名： | 张岭苓 李楠 徐凌川 秦国培 殷法杰 蒋海强 |
| 作者单位： | 湖南省白沙溪茶厂有限责任公司;山东中医药大学; |
| 摘 要： | 采用70%乙醇提取杜梨(Pyrus betulaefolia Bge.)叶中的总黄酮,探讨了以芦丁为对照品,Al(NO3)3为显色剂,分光光度法测定总黄酮的可行性。建立了NaNO2-Al(NO3)-NaOH与芦丁的反应体系,在510nm处测吸光度值,得回归方程Y=0.0024X+0.009,标准曲线相关系数r=0.9995,平均加样回收率98.1%,相对标准偏差0.98。在此条件下,不同炮制方法所得杜梨叶总黄酮含量测定结果分别为:日晒夜露传统制法8.32%,渥堆变红后日晒法6.45%。实验证明:杜梨叶总黄酮含量较高,可作为富含黄酮的药物资源加以开发利用,且从总黄酮含量看,传统的日晒夜露法优于渥堆变红加工法。 |
| 关 键 词： | 杜梨叶 总黄酮 分光光度法 含量测定 |
Determination of total flavonoids in leaves of Pyrus betulaefolia Bge.from different processing methods |
|
| ZHANG Ling-ling,LI Nan,XU Ling-chuan,QIN Guo-pei,YIN Fa-jie,JIANG Hai-qiang. Determination of total flavonoids in leaves of Pyrus betulaefolia Bge.from different processing methods[J]. Food Science and Technology, 2011, 0(10): 269-271 | |
| Authors: | ZHANG Ling-ling LI Nan XU Ling-chuan QIN Guo-pei YIN Fa-jie JIANG Hai-qiang |
| Affiliation: | ZHANG Ling-ling1,LI Nan1,XU Ling-chuan2,QIN Guo-pei2,YIN Fa-jie2,JIANG Hai-qiang2(1.Hunan Provincial Baishaxi Tea Industry Co.,Anhua 413515,2.Shandong University of Traditional Chinese Medicine,Jinan 250355) |
| Abstract: | The total flavonoids was extracted from the leaves of Pyrus betulaefolia Bge.with 70% ethanol,to discuss the feasibility of Al(NO3)3 as colour reagent with rutin as reference substance for evaluation on the content of total flavonoids by spectrophotometry.To establish a reaction between the rutin with the system of NaNO2-Al(NO3)3-NaOH,measure absorbance at 510 nm by spectrophotometer,The regression equation was Y=0.0024X+0.009;the correlation coeficient of standard curve was 0.9995,the average recovery was ... |
| Keywords: | Leaves of Pyrus betulaefolia Bge. total flavonoids spectrophotometry content determination |
| 本文献已被 CNKI 等数据库收录！ | |