GC法对中药材中残留农药的多残留分析

建立了中药材中8种有机氯农药和4种拟除虫菊酯农药残留气相色谱分析方法。样品用丙酮-石油醚超声提取,再用正已烷进行液-液分配,提取液用弗罗里硅土柱净化,采用SE-54弹性石英毛细管柱分离,用GC-ECD同时检测。测定结果高、中、低3个水平添加时的回收率为80.6%~119.8%,相对标准偏差为0.7%~9.5%。该方法的检出限为:有机氯农药0.0005~0.007mg/kg,拟除虫菊酯0.005~0.008 mg/kg。通过实验结果显示本方法可用于中药材中农药残留测定。

Determination of Organochlorine and Pyrethroid Pesticide Residues in Chinese Herbal Medicines by Capillary Cas Chromatography

Method to determine organochlorine and pyrethroid pesticide residues in Chinese herbal medicines by GC was found.Pesticide residues were extracted from Chinese herbal medicines with acetone and petroleum ether.Liquid-liquid extraction was performed with n-hexane and the clean-up of the extracts were made with alumina neutral-florisil column.Analytical screening was determined by gas chromatography using capillary column equipped with electron-capture detector.The recovery obtained from Chinese herbal medicines samples(n=3) at three concentration levels was in the ranges of 80.6%-119.8%,with relative standard deviations of 0.7%-9.5%.Detection limit was from 0.00005 to 0.0007 mg/kg for organochlorines and 0.005 to 0.008mg/kg for pyrethroids respectively.The results show that this method can be used to determine organochlorine and pyrethroid residues in Chinese herbal medicines.