Association of aerobic anoxygenic phototrophs and zebra mussels,Dreissena polymorpha,within the littoral zone of Lake Winnipeg |
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| Authors: | Steven Brady Kuzyk Kaitlyn Wiens Xiao Ma Vladimir Yurkov |
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| Affiliation: | 1. U.S. Geological Survey, Great Lakes Science Center, Tunison Laboratory of Aquatic Science, 3075 Gracie Road, Cortland, NY 13045, USA;2. U.S. Geological Survey, New York Cooperative Fish and Wildlife Research Unit, Cornell University, Ithaca, NY 14853, USA;3. SUNY Research Foundation, 3075 Gracie Road, Cortland, NY 13045, USA;1. Department of Fisheries and Wildlife, 480 Wilson Road, 13 Natural Resources Building, Michigan State University, East Lansing, MI 48864, United States;2. Department of Microbiology and Molecular Genetics, 567 Wilson Road, 2215 Biomedical Physical Sciences Building, Michigan State University, East Lansing, MI 48864, United States;3. Department of Integrative Biology, 288 Farm Ln, Natural Sciences Building, Michigan State University, East Lansing, MI 48864, United States;1. Environment and Climate Change Canada, Watershed Hydrology and Ecology Research Division, Victoria, BC V8W 3R4, Canada;2. Department of Civil Engineering, University of Manitoba, Winnipeg, MB R3T 5V6, Canada;3. University of Victoria, Department of Geography, Victoria, BC V8P 5C2, Canada;4. Environment and Climate Change Canada, Watershed Hydrology and Ecology Research Division, Saskatoon, SK S7N 1K2, Canada;5. Department of Food, Agricultural and Biological Engineering, the Ohio State University, Columbus, OH 44691, USA;6. Department of Geography, University of Calgary, ESB 458, 2500 University Drive NW, Calgary, AB T2N 1N4, Canada |
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| Abstract: | While nutrient loading has affected all levels of Lake Winnipeg’s ecology, its greatest influence has likely been on the microbial community. In addition to eutrophication, zebra mussels (Dreissena polymorpha) have recently invaded the ecosystem and threaten food web dynamics. Their filter-feeding predation and association with bacteria, specifically phototrophs, was investigated. A sampling trip to Lake Winnipeg in October 2017, focused on the isolation, enumeration, and identification of aerobic anoxygenic phototrophs in littoral water, sediment, and tissues of mussels. Gimli, Patricia, and Grand beaches, separated by >15 km across the South Basin, had similar bacterial counts when cultivated on rich organic, BG-11, purple non-sulphur, and K2TeO3-supplemented media. Culture-based enumeration on rich organic medium revealed 1.74% of heterotrophs from littoral waters were aerobic anoxygenic phototrophs, and represented 13.98% within sediments. In contrast, 0.48, 1.15, and 0.16% of cultured heterotrophs were aerobic anoxygenic phototrophs within zebra mussel gill, gut, and gonadal tissues, respectively. High-throughput sequencing of bacterial 16S V4 rDNA maintained similar trends in respective bivalve organs, where 0.22, 1.13, and 0.20% of total 16S genes belonged to these phototrophs. Several Sphingomonadaceae isolates were recovered from gut tissues, all with filamentous morphology large enough for predation. Bioaccumulation of metals was also studied in D. polymorpha. All tested associated aerobic anoxygenic phototrophs were capable of resisting the metalloid oxide tellurite. The consistent distribution of aerobic anoxygenic phototrophs within microbial communities across Lake Winnipeg, and their predominance in the gut tissues of zebra mussels suggested bacterial consumption by this invasive species. |
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| Keywords: | Aerobic anoxygenic phototrophs Microbiome Zebra mussel Metalloid oxides Lake Winnipeg |
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