Molecular sexing of lake sturgeon |
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| Authors: | Kim T. Scribner Jeannette Kanefsky |
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| Affiliation: | 1. Department of Fisheries and Wildlife, Michigan State University, 480 Wilson Rd, East Lansing, MI 48824, USA;2. Department of Integrative Biology, Michigan State University, 252 Farm Ln., East Lansing, MI 48824, USA;3. Ecology, Evolution, and Behavior Program, 103 Giltner Hall, 293 Farm Lane, East Lansing, MI 48824, USA;1. U.S. Fish and Wildlife Service, Lower Mississippi River Fish and Wildlife Conservation Office, 111 E. Elizabeth St., Tupelo, MS 38804, United States;2. U.S. Fish and Wildlife Service, Ashland Fish and Wildlife Conservation Office, 2800 Lake Shore Dr. E. Ashland, Wisconsin 54806, United States;1. Centre for Earth Observation Science, University of Manitoba, Winnipeg, MB, Canada;2. Department of Earth, Ocean and Atmospheric Sciences, University of British Columbia, Vancouver, BC, Canada;3. Department of Botany, University of British Columbia, Vancouver, BC, Canada;1. Centre for Earth Observation Science, Department of Environment and Geography, University of Manitoba, 125 Dysart Road, Winnipeg, Manitoba R3T 2M6, Canada;2. Freshwater Institute, Fisheries and Oceans Canada, 501 University Crescent, Winnipeg, Manitoba R3T 2N6, Canada;1. Rubenstein Ecosystem Science Laboratory, University of Vermont, Burlington, VT 05401, United States;2. Wisconsin Cooperative Fishery Research Unit, College of Natural Resources, University of Wisconsin – Stevens Point, 800 Reserve St. Stevens Point, WI 54481, United States;3. U.S. Geological Survey, Great Lakes Science Center, 1451 Green Road, Ann Arbor, MI 48105, United States;4. U.S. Geological Survey, Wisconsin Cooperative Fishery Research Unit, College of Natural Resources, University of Wisconsin‐Stevens Point, 800 Reserve St. Stevens Point, WI 54481, United States;5. National Oceanographic and Atmospheric Administration, National Marine Fisheries Service, Alaska Fisheries Science Center, Auke Bay Laboratories, 17109 Point Lena Loop Road, Juneau, AK 99801, United States;6. U.S. Geological Survey, Great Lakes Science Center, Lake Superior Biological Station, Ashland, WI 54806, United States |
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| Abstract: | Demographic data including characterizations of population sex and age composition are of fundamental importance for effective management, especially for numerically depressed Great Lakes populations of imperiled species such as lake sturgeon (Acipenser fulvescens). The goal of this paper was to extend a recently reported Acipenserid-derived PCR-based genotyping test to determine sex of lake sturgeon. We demonstrate that the recently reported AllWSex2 primers amplified the female sex-specific region in lake sturgeon of known sex, consistent with a ZZ/ZW mode of inheritance. Sanger sequencing of female lake sturgeon PCR products matched published sequences from female sterlet (Acipenser ruthenus), showing 100% query cover and 98% (63/64) identity. The ability to provide a rapid, cost-effective, and unambiguous determination of sex for lake sturgeon will allow managers to determine compositional estimates of sex ratios during any season, and for individuals at any age or size, which is of great utility for species characterized by delayed sexual maturity and lacking external sexual dimorphisms. |
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| Keywords: | Lake sturgeon Molecular sex determination |
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