| 液相色谱在生物大分子分离研究中的应用 |
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| 引用本文： | 谷春秀,赵伟,于志勇,谷雪枫,徐宏博.液相色谱在生物大分子分离研究中的应用[J].生命科学仪器,2012,10(2):40-44. |
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| 作者姓名： | 谷春秀 赵伟 于志勇 谷雪枫 徐宏博 |
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| 作者单位： | 北京联合大学生物化学工程学院,北京,100023 |
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| 摘 要： | 本文讨论了液相色谱法在生物大分子分离过程中的应用,主要探讨色谱柱的选择以及分离条件的确定。以糖类为例介绍了液相色谱分离生物分子的具体方法,同时对柱温流动相等因素进行分析和讨论。液相色谱法应用于生物大学子实验教学,对学生掌握先进的分离方法具有很重要的意义。
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| 关 键 词： | 生物大分子 糖类 液相色谱法 |
Application of Liquid Chromatography in The course of Biological Macromolecular |
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| Gu Chunxiu,Zhao Wei,Yu Zhiyong,GuXuefeng,Xu Hongbo.Application of Liquid Chromatography in The course of Biological Macromolecular[J].Life Science Instruments,2012,10(2):40-44. |
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| Authors: | Gu Chunxiu Zhao Wei Yu Zhiyong GuXuefeng Xu Hongbo |
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| Affiliation: | (Biochemical Engineering College of Beijing Union University,Beijing 100023,China) |
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| Abstract: | In this study,the application of liquid Chromatography in biological macromolecular separation was discussed.The factors discussed indicate the selection of the column and condition for separation.The carbohydrate r are taken as experimental sample an influent factors,such as temperature of column and different elution were observed an analyzed.It was conclusion that Liquid Chromatography can be used in the course of macromolecular separation to let our students master some advanced separation method. |
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| Keywords: | Biological macromolecular Carbohydrate Liquid Chromatography |
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