| 枯草芽孢杆菌BSD-2产抗菌肽发酵培养基的优化 |
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| 引用本文： | 张蕾,张铎,张丽萍,程辉彩,胡瑞萍,张根伟.枯草芽孢杆菌BSD-2产抗菌肽发酵培养基的优化[J].食品科学,2010,31(3):189-192. |
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| 作者姓名： | 张蕾 张铎 张丽萍 程辉彩 胡瑞萍 张根伟 |
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| 作者单位： | 1.河北省科学院生物研究所 2.河北师范大学生命科学学院 3.河北化工医药职业技术学院 |
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| 基金项目： | 河北省科技支撑计划项目(09220304D); 河北省财政资金项目(07305); 河北省自然科学基金项目(C2009001280) |
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| 摘 要： | 为了提高枯草芽孢杆菌BSD-2抗菌肽的产量，应用响应面法对发酵培养基进行优化。采用Plackett-Burman设计对培养基中相关影响因素的效应进行评价，筛选出3个重要因素依次为蛋白胨、淀粉和豆饼粉；然后进行最陡爬坡试验逼近最佳响应面区域；最后通过Box-Behnken设计及响应面分析法确定最佳培养基配方为：蛋白胨14.29g/L、淀粉14.07g/L、豆饼粉6.49g/L、CaCO3 2.0g/L、MgSO4 1.0g/L。拟合实验模型结果显示，发酵液抗菌肽的产量增加为原来的1.77倍。
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| 关 键 词： | 枯草芽孢杆菌 响应面分析 Minitab软件 培养基优化 抗菌肽 |
| 收稿时间： | 2009-04-21 |
Optimization of Fermentation Medium for Production of Antibacterial Peptides by Bacillus subtilis BSD-2 |
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| ZHANG Lei,ZHANG Duo,ZHANG Li-ping,CHENG Hui-cai,HU Rui-ping,ZHANG Gen-wei.Optimization of Fermentation Medium for Production of Antibacterial Peptides by Bacillus subtilis BSD-2[J].Food Science,2010,31(3):189-192. |
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| Authors: | ZHANG Lei ZHANG Duo ZHANG Li-ping CHENG Hui-cai HU Rui-ping ZHANG Gen-wei |
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| Affiliation: | 1. Institute of Biology, Hebei Academy of Science, Shijiazhuang 050081, China 2. College of Life Science, Hebei Normal
University, Shijiazhuang 050016, China 3. Hebei Chemical and Pharmaceutical College, Shijiazhuang 050026, China |
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| Abstract: | Plackett-Burman design was used to evaluate the importance of fermentation medium factors of Bacillus subtilis BSD-2 affecting the inhibitory zone diameter of produced antibacterial peptides.Subsequently,7 steepest ascent experiments involving 3 most important factors(i.e.,peptone,starch and soybean meal) were conducted for approaching their optimal response region.Finally,the optimal fermentation medium formula was obtained by optimizing the most important factors using central composite design combined wi... |
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| Keywords: | Bacillus subtilis response surface methodology Minitab software medium optimization antibacterial peptide |
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