| DABS-Cl 试剂在反相高效液相色谱中的应用 |
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| 引用本文： | 费达,江国荣,李勇健,任光荣.DABS-Cl 试剂在反相高效液相色谱中的应用[J].化学试剂,1993(1). |
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| 作者姓名： | 费达 江国荣 李勇健 任光荣 |
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| 作者单位： | 苏州大学分析测试中心 苏州215006(费达)，苏州中医研究所 苏州215003(江国荣，李勇健，任光荣) |
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| 摘 要： | 选定了两套完全不同的色谱条件,并对 DABS-氨基酸进行了高效液相色谱分析实验。DABS-Cl 与氨基酸的衍生反应所需的时间不足 lh。
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| 关 键 词： | DABS-Cl 反相高效液相色谱 衍生反应 DABS-氨基酸 |
Application of DABS-C(?) reagent In reverse-phase high performance liquid chromatography |
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| Fei Da.Application of DABS-C(?) reagent In reverse-phase high performance liquid chromatography[J].Chemical Reagents,1993(1). |
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| Authors: | Fei Da |
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| Abstract: | The DABS-Cl(4-dimethylaminoazobenzene-4'-sulf-onyl chloride)is a sort of chromophoric labelling reagent em-ployed for labelling of amino acids,polypeptides,and pro-teins.With the DABS-Cl precolumn derivatization techni-que,the above mentioned compounds are easily separated byhigh performance liquid chromatography,and then deter-mined spectrophotometrically at 436nm at the picomole(10~(-12)mol)level.The DABS derivatives are highly stable,the derivatization procedure is relatively simple and fairlyrapid,the whole procedure takes less than one hour. |
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| Keywords: | DABS-Cl reverse-phase high performance liquid chromatography derivatization technique amino acid |
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