Guinea pig bone marrow cells treated with platelet-activating factor generate factor(s) which affects their DNA synthesis and microbicidal activity |
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Authors: | Ichiro Kudo Toshiyuki Kato Hidetoshi Hayashi Ryohei Yanoshita Koichi Ikizawa Hiroko Uda Keizo Inoue |
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Affiliation: | (1) Faculty of Pharmaceutical Sciences, University of Tokyo, Hongo, Bunkyo-ku, 113 Tokyo, Japan;(2) Present address: Shionogi Research Laboratories, Shionogi & Co., Ltd., 553 Osaka, Japan;(3) Present address: Faculty of Pharmaceutical Sciences, Nagoya City University, 467 Nagoya, Japan;(4) Present address: Technical R&D Division, Terumo Corporation, 259-01 Kanagawa, Japan;(5) Present address: Biological Research Laboratory, Taiho Pharmaceutical Corporation Ltd., 771-01 Tokushima, Japan |
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Abstract: | We have previously reported that platelet-activating factor (PAF) induces proliferation and microbicidal activity of guinea
pig bone marrow cells. In the present study, we have found that the conditioned medium of PAF- or nonmetabolizable PAF agonist-treated
guinea pig bone marrow cells augmented DNA synthesis and induced microbicial activity of bone marrow cells. A PAF specific
antagonist, CV-6209, inhibited generation of the active conditioned medium by PAF. Addition of the PAF antagonist only partially
suppressed the augmentative effect of the active conditioned medium on DNA synthesis; this is consistent with the fact that,
because of the rapid breakdown, no appreciable amount of PAF remained in the conditioned medium of PAF-treated cells. Although
mouse bone marrow cells did not respond to PAF unlike guinea pig cells, their DNA synthesis was significantly enhanced by
the conditioned medium of PAF-treated guinea pig bone marrow cells. Thus, some newly generated factor(s) distinct from the
originally inoculated PAF seemed to modulate the bioactions of PAF on bone marrow cells. An appreciable amount of PAF was
produced by calcium ionophore-treated guinea pig bone marrow cells. These findings indicate that PAF synthesized in guinea
pig bone marrow cells induces generation in the cells of some factor(s) which affects proliferation or microbicidal activity.
Presented at The Third International Conference on Platelet-Activating Factor and Structurally Related Ether Lipids, Tokyo,
Japan, May 1989. |
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