化学计量学二阶校正法结合三维荧光光谱技术同时快速测定人血清样中西酞普兰、氟西汀和帕罗西汀含量

应用化学计量学二阶校正方法结合三维荧光光谱技术可以实现人血清样中西酞普兰、氟西汀和帕罗西汀含量的直接、快速同时测定。设定荧光激发波长范围为210²60 nm,发射波长为270260 nm,发射波长为270³88 nm,测定不同样本的三维荧光光谱,构建三维响应数阵。当选取体系组分数为4时,采用PARAFAC方法得到的血清样中西酞普兰、氟西汀和帕罗西汀的加标回收率(95%置信区间)分别为95.9±3.22%、104.5±8.17%和96.4±5.15%,并采用品质因子和椭圆置信区间测试对结果进行了进一步验证。实验结果表明,该方法快速简便,解析结果可靠,无需样本预处理,能直接用于同时分析人血清中西酞普兰、氟西汀和帕罗西汀的含量。

Simultaneous and Rapid Determination of Citalopram,Fluoxetine and Paroxetine Contents in Human Serum Samples Using Three -dimensional Fluorescence Technique Coupled with Chemometric Second order Calibration

A novel and effective approach was developed for the simultaneous determination of citalopram（CIT）,fluoxetine（FLU） and paroxetine（PAR） in human plasma samples by the incorporation of excitationemission fluorescence（EEM） and second-order calibration method based on the parallel factor analysis（PARAFAC） algorithm.The scanning range of their excitation and emission wavelengths were set at 210～260 nm and 270～380 nm,respectively.The obtained 95% confidence interval of CIT,FLU and PAR in human serum samples by using PARAFAC method with an estimated component number of four were 95.9 ±3.22,104.5±8.17 and 96.4±5.15%,respectively.Moreover,the accuracy of the method was also evaluated through elliptical joint confidence region（EJCR） tests as well as the figures of merit.The experimental results demonstrated that the method has been satisfactorily applied to determination of CIT,FLU and PAR in human serum samples without previous separation procedure.