毛蕊花糖对巨噬细胞RAW264.7免疫调节作用的研究

目的：明确毛蕊花糖对巨噬细胞RAW264.7免疫功能的调节作用。方法：分别以质量浓度25、50、100、200、400μg/mL的毛蕊花糖对RAW264.7巨噬细胞进行培养，以10μg/mL的脂多糖(LPS)为阳性对照组、不加任何样品的培养基为空白对照组。通过噻唑蓝(MTT)实验、吞噬中性红实验、NO释放量测定及酶联免疫吸附法测定细胞因子(IL-6、IL-1β、IFN-α、IFN-γ)的分泌量，评价毛蕊花糖的免疫调节作用。结果：质量浓度为25～400μg/mL的毛蕊花糖对巨噬细胞RAW264.7作用24h后，可显著提高巨噬细胞的增殖活性、吞噬活性、NO释放量以及细胞因子的分泌水平，并且毛蕊花糖溶液质量浓度为200μg/mL时效果最好。结论：毛蕊花糖对小鼠腹腔巨噬细胞系RAW264.7具有免疫调节活性，是一种良好的免疫增强剂。

Immunoregulatory Function of Verbascose on Macrophage Cell Line RAW264.7

Objective: To investigate the immunoregulatory function of verbascose on macrophage cell line RAW264.7. Methods: RAW264.7 cells were cultured in a 96-well plate with different concentrations of verbascose (25, 50, 100, 200 μg/mL and 400 μg/mL), and the role of verbascose was evaluated based on the proliferative activity of cultured splenocytes as determined by MTT assay, nitric oxide (NO) production and cytokines (IL-6, IL-1β, IFN-α and IFN-γ) expression levels, neutral red phagocytosis. In the present study, lipopolysaccharide (LPS, 10 μg/mL) was used as a positive control, and treatment without verbascose or LPS was used as a negative control. Results: Verbascose significantly improved the proliferation, phagocytosis, NO level and cytokines expression levels when RAW264.7 cells were cultured for 24 h in the studied concentration range. Verbascose at a dose of 200 μg/mL showed the highest effect. Conclusion: Verbascose has an immunomodulatory effect on macrophage cell line RAW264.7 as a potential immunomodulatory enhancer.