低能氮离子注入和紫外线复合诱变选育高产酿酒酵母

通过离子注入联合紫外线辐射诱变选育高产酿酒酵母菌株,根据存活率及突变率确定离子注入的诱变条件为电荷18 ke V,剂量400×10~(14) cm~(-2),紫外线辐射60 s。在该条件下,诱变获得的菌株正突变率和致死率都最高,诱变效果最好。在该条件下进行研究后获得目标菌株SC-N9015,酒精产量从14.65%提升到15.53%。多次传代实验结果显示遗传性状稳定。研究表明,通过低能氮离子注入和紫外线联合诱变选育高产酒精酵母效果显著。

Mutation breeding of ethanol-producing yeast Saccharomyces cerevisiae by low-energy nitrogen ion implantation and UV rays

The ethanol-producing yeast Saccharomyces cerevisiae was induced by N+ implantation with an output power of 18 keV and a dose of 400×1014 cm?2,depending on the survival and mutation rates. The UV exposure time was 60 s. Under optimized conditions, the strain achieved the highest positive mutation and lethality rates. A mutant strain SC-N9015 was selected; the ethanol yield of this strain was 15.53%, whereas that of the parental strain was 14.65%. Further, evaluation of ethanol yield over successive generations indicated the hereditary stability of the mutations. The study shows that the combination of low energy N+implantation with UV rays is an efficient method for mutation breeding of S. cerevisiae.