| 平贝母多糖的分离纯化及抗氧化活性研究 |
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| 引用本文： | 刘春红,马宇,何忠梅,韩宝瑞.平贝母多糖的分离纯化及抗氧化活性研究[J].食品科学,2011,32(21):29-33. |
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| 作者姓名： | 刘春红 马宇 何忠梅 韩宝瑞 |
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| 作者单位： | 1.长春大学特殊教育学院
2.吉林农业大学发展学院
3.吉林农业大学中药材学院 |
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| 基金项目： | 吉林省教育厅“十二五”科学技术研究项目(吉教科合字2011第210号) |
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| 摘 要： | 采用乙醇沉淀、DEAE-纤维素离子交换层析和Sepharose CL-6B凝胶过滤层析法，从平贝母(Fritillaria ussuriensis Maxim)水提取液中分离纯化得到水溶性多糖，命名为FUP-1。通过红外光谱和高效液相色谱对其结构和单糖组成进行初步分析，分子筛层析法测定其分子质量。结果表明，FUP-1为均一组分的杂多糖，主要由木糖、葡萄糖和半乳糖组成，其物质的量比为5:1:1，平均分子质量为4.1×104D。体外抗氧化活性分析表明，FUP-1具有较强的清除羟自由基、超氧阴离子自由基和DPPH自由基的能力。
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| 关 键 词： | 平贝母 多糖 单糖组成 抗氧化 |
Partial Characterization and Antioxidant Activity of Water-soluble Polysaccharide Isolated from Bulbus Fritillariae ussuriensis |
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| LIU Chun-hong,MA Yu,HE Zhong-mei,HAN Bao-rui.Partial Characterization and Antioxidant Activity of Water-soluble Polysaccharide Isolated from Bulbus Fritillariae ussuriensis[J].Food Science,2011,32(21):29-33. |
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| Authors: | LIU Chun-hong MA Yu HE Zhong-mei HAN Bao-rui |
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| Affiliation: | (1. Special Education College, Changchun University, Changchun 130022, China；
2. Development College, Jilin Agricultural University, Changchun 130600, China；
3. College of Chinese Medicine Materials, Jilin Agricultural University, Changchun 130116, China) |
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| Abstract: | A water-soluble polysaccharide was isolated from Bulbus Fritillariae ussuriensis(dried bulbs of Fritillaria ussuriensis Maxim) sequentially through water extraction,ethanol precipitation,ion exchange chromatography and gel permeation chromatography.The molecular mass of FUP-1 was determined by gel filtration chromatography,and its structure was characterized by HPLC and FTIR spectroscopy.The results showed that FUP-1 was a heteropolysaccharide,of which the monosaccharide composition was composed of xylose,g... |
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| Keywords: | Fritillaria ussuriensis polysaccharide monosaccharide composition antioxidant activity |
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