| ZnO-PDMS超双疏薄膜对腐败希瓦氏菌生物被膜的抑制机制 |
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| 作者姓名： | 黄海涛 魏旭青 杨峻乙 黄艳茹 王莉丽 杨旭 励建荣 孙彤 |
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| 作者单位： | 渤海大学 食品科学与工程学院/海洋食品精深加工关键技术省部共建协同创新中心/生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心, 辽宁 锦州 121013;渤海大学 食品科学与工程学院/海洋食品精深加工关键技术省部共建协同创新中心/生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心, 辽宁 锦州 121013;成都先导药物开发股份有限公司, 四川 成都 610000;民泽龙羊峡生态水殖有限公司, 青海 海南州 811800 |
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| 基金项目： | 国家重点研发计划蓝色粮仓科技创新重点专项(2019YFD0901702)； 辽宁省科技特派行动专项计划项目(2021030202-JH5/104)。 |
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| 摘 要： | 为提高食品贮藏加工材料表面对水产品腐败希瓦氏菌生物被膜的抗黏附性能,以聚二甲基硅氧烷(PDMS)为成膜剂,以水热合成法制备的不同微观形貌的ZnO微纳米粒子为改性剂,采用流延法制备了ZnO-PDMS薄膜,并研究了薄膜对腐败希瓦氏菌生物被膜的抑制性能及作用机理。结果表明:改性ZnO微纳米粒子的微观形貌影响PDMS薄膜的疏水疏油性能,以绒球状和花状ZnO微纳米粒子为改性剂,可获得ZnO-PDMS超双疏薄膜,对水和油的接触角均大于150°。薄膜的抗菌黏附性能与疏水疏油性能呈正相关,随着培养时间的延长,ZnO薄膜释放的Zn2+破坏了细菌细胞膜,并抑制胞内ATP酶和AKP酶活性,引起细菌代谢紊乱导致细菌死亡。相较于棒状和绒球状微纳米粒子改性的PDMS薄膜,花状ZnO-PDMS薄膜具有更强的抗黏附性能和抑菌效能。同时具有超双疏性能和抗菌性能的ZnO-PDMS薄膜可有效抑制腐败希瓦氏菌生物被膜的形成和生长,研究旨在为抗生物被膜材料的研制提供技术支持。
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| 关 键 词： | ZnO 聚二甲基硅氧烷 超双疏薄膜 腐败希瓦氏菌 生物被膜 胞外多糖 ATP酶 |
| 收稿时间： | 2022/4/22 0:00:00 |
Antibiofilm Mechanism of ZnO-PDMS Superamphiphobic Films Against Shewanella putrefaciens |
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| Authors: | HUANG Haitao WEI Xuqing YANG Junyi HUANG Yanru WANG Lili YANG Xu LI Jianrong SUN Tong |
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| Affiliation: | College of Food Science and Technology/Collaborative Innovation Center of Seafood Deep Processing, /National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Bohai University, Jinzhou 121013, China;College of Food Science and Technology/Collaborative Innovation Center of Seafood Deep Processing, /National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Bohai University, Jinzhou 121013, China;Chengdu Lead Drug Development Co Ltd, Chengdu 610000, China;Minze Longyangxia Aquaculture Co Ltd, Hainan Prefecture 811800, China |
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| Abstract: | To improve the surface anti-adhesion property of food storage and processing materials against Shewanella putrefaciens biofilm of aquatic products, the ZnO-PDMS films were prepared by tape casting method with polydimethylsiloxane (PDMS) as filmogen, ZnO micro-nanoparticles with different micro-nanophology prepared by hydrothermal synthesis method as modifiers, and antibiofilm property and mechanism of the ZnO-PDMS films against Shewanella putrefaciens biofilm were studied. The results showed that the hydrophobic and oleophobic properties of PDMS films were affected by micromorphology of modified ZnO micro-nanoparticles. Using pompon-shaped and flower-shaped ZnO micro-nanoparticles as modifiers, ZnO-PDMS superamphiphobic films were prepared, and their contact angles of water and oil were both beyond 150°. There was a positive correlation between antibacterial adhesion and amphiphobic of the films. With the extension of culture time, Zn2+ released from ZnO film, damaged the bacterial cell membrane and inhibited ATP and AKP enzyme activity in the cell, resulting in bacterial metabolic disorder and death. Compared with PDMS films modified by rod-shaped and pompon-shaped micro-nanoparticles, flower-shaped ZnO-PDMS film had stronger anti-adhesion and antibacterial property. Formation and growth of Shewanella putrefaciens biofilm could be effectively inhibited by the ZnO-PDMS film with both surper-amphiphobic and antibacrobic properties. This study aimed to provide technical support for development of antibiofilm materials. |
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| Keywords: | ZnO PDMS superamphiphobic film Shewanella putrefaciens biofilm exopolysaccharides ATP enzyme |
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