降胆固醇乳酸菌菌株的筛选及其影响因素的研究

选择由本实验室分离并保存的4种乳酸菌株,接种到10%脱脂乳培养基中进行活化和扩大培养。测量培养基在接种前后吸光度值的变化,从中筛选出较强降胆固醇能力的一株乳酸菌种,并考察在不同培养时间、接种量、培养温度、胆盐浓度、盐浓度、乳糖添加量下该菌种降胆固醇能力的变化。结果表明：胆酸盐和盐对该菌株的降胆固醇能力都有一定的抑制作用,胆酸盐的抑制作用高于盐的作用;对于培养条件来说,当培养温度为42℃,接种量为1%,培养时间为24h时,该菌株的降胆固醇能力最高。

Cholesterol-reducing Lactic Acid Bacteria Screening and Research

We choose four strains of lactobacillus which is separated and preserved by our lab,and activate them by culturing them in the 10% skim milk medium.Through measuring the change of the cholesterol of the skim milk,we judge the difference of the cholesterol-reducing ability of the lactic acid bacteria and select the best one.Then we study on the gallbladder salt,the salt,the lactose,the culture temperature,the culture time,and the inoculations on the cholesterol-reducing capability of the lactic acid bacteria.And the result shows that the salt and the gallbladder salt can restrain the cholesterol-reducing capability of it,and when the temperature is 42℃,the time is 24h,the inoculation is 1%,the cholesterol-reducing capability of the LAB is best.