甘草油食用安全性评价

通过小白鼠急性毒性试验、小白鼠精子畸形遗传毒性试验和亚急性毒性试验对甘草油的毒理学进行研究,并对其作出安全性评价。试验结果表明,甘草油对小白鼠的急性经口半数致死剂量LD 50>15.0 g/kg·BW,判属无毒类。小白鼠精子畸形遗传毒性试验为阴性,表明该受试物甘草油无致突变作用。在大白鼠30 d喂养试验中未见动物健康状况、生化、血液学指标和器官组织形态的异常变化,据此初步估计甘草油的最大无作用剂量>5.0 g/kg·BW,为人体推荐摄入量的150倍。说明甘草油无急性毒性、亚急性毒性和遗传毒性,具有较高的食用安全性。

Assessment of the edible safety of licorice oil

The toxicology of licorice oil was researched through the acute toxicity test of mice,genotoxicity test of mouse sperm deformity and subacute toxicity test,and made a safety evaluation on it.The experimental results showed that the acute oral LD 50 of licorice oil on mice was greater than 15.0 g/kg·BW,which was classified as non-toxic.The mouse sperm deformity genetic toxicity test was negative,indicating that the test substance had no mutagenic effect.No abnormal changes in animal health status,biochemistry,hematology indexes and organ tissue morphology were observed in the 30-day feeding experiment.Based on this,it was estimated that the maximum non-effective dose of the product was greater than 5.0 g/kg·BW,which was 150 times as high as the recommended human intake.Therefore,licorice oil had no acute toxicity,subacute toxicity and genotoxicity,and licorice oil was excellent edible oil with higher safety.