罗非昔布对胃癌细胞上清液中培养的树突状细胞分化成熟和功能的影响

目的：从免疫学角度分析罗非昔布（Rofecoxib）抗癌作用的机制。方法：将人外周血分离的单核细胞加入含重组人粒细胞巨噬细胞集落刺激因子和重组人白细胞介素的培养液中,随机分为空白组（PBS培养液）,干预组（胃癌细胞上清液＋罗非昔布,150mg/L）,对照组（胃癌细胞上清液）使其分化为树突状细胞（denstritic cell,DC）。混合培养48h后,动态观察DC成熟过程中的形态学变化,流式细胞术检测DC成熟表型（CD40、CD80）,混合T淋巴细胞增殖反应检测DC功能。结果：与对照组比较,空白组、干预组DC的CD40、CD80表达明显在增加（P〈0.05）,T细胞增殖作用明显增强（P〈0.05）。结论：罗非昔布可通过促进DC成熟,并增加DC的免疫活性而发挥抗癌作用。

Effect of rofecoxib on maturation and immune function of den-dritic cells in supernatants of gastric cancer

Objective：To study the effect of Rofecoxib on maturation and immune function of dendritic cells（DCs） in supernatants of gastric cancer.Methods：Immature DCs from peripheral blood mononuclear cells,were randomly divided into the blank group（dealt with PBS）,the intervention group（dealt with gastric cancer cell superrrnatant treated by Rofecoxib）,the control group（dealt with gastric cancer cell supernatant）,were cocultured with recombinant human granulocyte join macrophage colony-stimulating factor（rhGM-CSF） and recombinant human inter-leukin-2（rhIL-2） to differentiate into DC.The morphological changes of DCs were observed serially and the effect of Rofecoxib on the maturation of DC was analyzed by flow cytometer（analying DC mature phenotypes（CD40,CD80）.Allogeneic mixed T lymphocytes reaction and supernatants cytokine measurement were used for immune functional assay.Results：Compared with the control group,the intervention group and the blank group DCs upregulated CD40 and CD80 expression and promoted allogeneic T cells proliferation.Conclusion：Rofecoxib may play the important role in DC maturation and Anti-tumour immunity.