| LHRH-PE40对LOVO细胞的靶向杀伤作用 |
| |
| 引用本文： | 吴广谋,张国利,岳玉环,何畅,朱平.LHRH-PE40对LOVO细胞的靶向杀伤作用[J].粉末涂料与涂装,2006,19(4):349-351. |
| |
| 作者姓名： | 吴广谋 张国利 岳玉环 何畅 朱平 |
| |
| 作者单位： | 军事医学科学院军事兽医研究所生物技术应用室 长春130062 |
| |
| 摘 要： | 目的研究LHRH-PE40对LOVO细胞的靶向杀伤作用。方法用XTT检测LHRH-PE40对LOVO细胞的体外细胞毒性。将LOVO细胞移植于裸鼠,测定静脉注射LHRH-PE40对体内肿瘤细胞生长的抑制作用。结果LHRH-PE40对LOVO细胞细胞毒作用的半数抑制浓度为2·103μg/ml,在裸鼠肿瘤模型中,LHRH-PE40剂量为150μg/kg时抑瘤率为68·49%,这种细胞毒性作用可以被LHRH(或LHRH类似物)特异性抑制。结论在体内外LHRH-PE40对LOVO细胞具有很强的细胞毒作用,其机制是LHRH-PE40通过与癌细胞膜上的LHRH受体特异结合发挥细胞杀伤作用。
|
| 关 键 词： | LHRH-PE40 XTT 细胞毒性 LOVO细胞系 |
| 收稿时间： | 2005-12-08 |
| 修稿时间： | 2005年12月8日 |
Targeting Cytotoxicity of LHRH-PE40 to LOVO Cells |
| |
| WU Guang-mou ,ZHANG Guo-li, YUE Yu-huan ,et al.Targeting Cytotoxicity of LHRH-PE40 to LOVO Cells[J].Chinese Journal of Biologicals,2006,19(4):349-351. |
| |
| Authors: | WU Guang-mou ZHANG Guo-li YUE Yu-huan |
| |
| Affiliation: | Institute of Military Veterinary,Academy of Military Medical Sciences, Changchun 130062, China |
| |
| Abstract: | Objective To study the targeting cytotoxicity of LHRH-PE40 to LOVO cells.Methods Determine the cytotoxicity in vitro of LHRH-PE40 to LOVO cells by XTT method.Transplant LOVO cells to nude mice,then inject the mice intravenously with LHRH-PE40 and determine the inhibiting rate to growth of LOVO cells.Results The 50% inhibition concentration of LHRH-PE40 to LOVO cells was 2.103 μg/ml.The inhibiting rate of LHRH-PE40 at a dosage of 150 μg/kg to the growth of LOVO cells was 68.49%.The cytotoxicity was specifically inhibited by LHRH or LHRH analogue.Conclusion LHRH-PE40 showed strong cytotoxicity to LOVO cells by specific binding to the LHRH receptor on membrane of the cells. |
| |
| Keywords: | LHRH-PE40 XTT Cytotoxicity LOVO cell line |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
