Microplate hybridization for Borna disease virus RNA in human peripheral blood mononuclear cells |
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| Authors: | S Fujiwara H Takahashi T Nakaya Y Nakamura K Nakamura K Iwahashi H Kazamatsuri S Iritani N Kuroki K Ikeda K Ikuta |
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| Affiliation: | Section of Serology, Institute of Immunological Science, Hokkaido University, Kita-ku, Sapporo, Japan. |
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| Abstract: | ![]()
We developed a simple and sensitive microplate hybridization procedure with which to identify Borna disease virus cDNA in amplified products from human peripheral blood mononuclear cells. The mean values for the positive PCR products were significant compared with those for any of the negative products, indicating that this method can be applied to rapidly diagnose a large number of clinical specimens. |
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