乙醇对小鼠免疫系统影响的研究

为给评价声称具有提高免疫的能力的含乙醇保健食品提供依据 ,观察不同剂量乙醇对小鼠免疫系统的影响。采用 17～ 2 0g的雄性BalB c小鼠 90只 ,随机分为 6组 ,每组 15只 ,分别以 0、0 6 7、1 33、3 33、6 0 8、8 0 0g kgBW 乙醇每天一次灌胃给予 ,灌胃量为 2 0mL kgBW,连续灌胃 30d后 ,分别测定细胞免疫、体液免疫、单核巨噬细胞及NK细胞活性等 7项指标。结果表明 :(1)乙醇可以导致小鼠的免疫系统损伤。 (2 ) 8 0 0g kgBW(5 0 % )剂量组在一次灌胃后 2 0min内全部进入昏睡状态 ,2 4h内全部死亡。 (3) 6 0 8g kgBW(38% )组小鼠死亡率为 82 2 %。存活小鼠碳粒廓清能力低下 ,至 30d时仍未恢复。 (4 ) 1 33g kgBW(8% )组腹腔巨噬细胞吞噬鸡红细胞能力 (吞噬率 )降低 ,但对鸡红细胞吞噬指数无显著性影响。 (5 ) 0 6 7g kgBW(4 % )组NK细胞活性降低。 (6 ) 3 33g kgBW(2 0 % )组小鼠的 7项免疫指标均无显著性影响。 (7)以酒精为载体的保健食品在进行免疫调节作用评价实验时 ,小鼠灌胃液中酒精的浓度不宜超过 2 0 %。

Study on alcohol damage to immune system in Mice

To observe the damage to immune system in mice,alcohol of different concentration was administered to 6 groups of male BalB/c mice by gavage in doses of 0,0.67(4%),1.33(8%),3.33(20%),6.08(38%),8.00(50%)g/kg BW once a day respectively for 30 days. The functions of the immune system including the cellular immunity,the humoral immunity,the nonspecific immunity,and the NK cell activity were examined. The results showed that: 1) the alcohol can cause damage to the immune system of the mice,2) all mice of the 8.00 g/kg BW group died within 24 hours after the first gavage;3) in the 6.08g/kg BW group,the mortality within 30 days was 82%,and the ability to sweep carbon particles off the blood was depressed;4) the phagocytosis was depressed in the mice of the 1.33g/kg BW group;5)the NK cell activity was depressed in the mice of the 0.67g/kg BW group;6) no statistically significant change in the seven immune system functions was observed in the 3.33g/kg BW group;7) the concentration of alcohol in a health food must lower than 20% when assessing its effect on immunity experimentally with mice.