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产细菌素的戊糖片球菌的筛选及其细菌素的理化性质研究
引用本文:张红星,刘丽,谢远红,熊丽霞,刘慧.产细菌素的戊糖片球菌的筛选及其细菌素的理化性质研究[J].现代食品科技,2011,27(2):135-138,143.
作者姓名:张红星  刘丽  谢远红  熊丽霞  刘慧
作者单位:北京农学院食品科学系,北京,102206
摘    要:从牛肉丸中分离筛选到1株具有抑菌活性的乳酸菌菌株N463,对单核细胞增生李斯特菌ATCC54003的生长具有良好的抑制作用.16S rDNA序列同源性分析鉴定乳酸菌N463为戊糖片球菌.在排除有机酸、过氧化氢的干扰后,确定该抑菌物质为蛋白类物质,即细菌素,命名为细菌素N463.理化性质研究表明细菌素N463具有较好的热...

关 键 词:细菌素  戊糖片球菌  筛选  理化性质

Screening of Bacteriocin-producing Pediococcus pentosaceus Strain and Study on Physicochemical Characteristics of Bacteriocin
ZHANG Hong-xing,LIU Li,XIE Yuan-hong,XIONG Li-xia,LIU Hui.Screening of Bacteriocin-producing Pediococcus pentosaceus Strain and Study on Physicochemical Characteristics of Bacteriocin[J].Modern Food Science & Technology,2011,27(2):135-138,143.
Authors:ZHANG Hong-xing  LIU Li  XIE Yuan-hong  XIONG Li-xia  LIU Hui
Affiliation:(Department of Food Science,Beijing University of Agriculture,Beijing 102206,China)
Abstract:Strain N463,isolated from beef balls,produced antimicrobial substance which was inhibitory to Listeria monocytogenes 54003. This strain was identified as Pediococcus pentosaceus by using 16S rDNA gene sequence homology analysis.After eliminating the effect of organic acid and hydrogen peroxide,the antimicrobial substance with its proteinaceous nature was confirmed as bacteriocin.Bacteriocin N463 was heat-stable and remained activity after incubation within wide pH range.It could be degraded by proteases existing in the human body.This preliminary study on the bacteriocin produced by Pediococcus pentosaceus laid the basis for the development of natural meat products preservative.
Keywords:bacteriocin  Pediococcus pentosaceus  screening  physicochmical characteristics
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