系统性淀粉样变性的超微病理诊断

目的:应用光镜(LM)及透射电镜(TEM)观察不同部位淀粉样物质的沉积及超微结构特点,为系统性淀粉样变性提供可靠的病理诊断依据.方法:对21例临床拟诊为系统性淀粉样变性病例的肾脏,腹壁脂肪和直肠粘膜活检组织按常规进行LM及TEM标本制备及病理检查.结果:LM观察显示淀粉样物质在肾脏,腹壁脂肪和直肠粘膜沉积阳性率分别为7...

The ultrapathological diagnosis of systemic amyloidosis

Objective： To observe the histological and ultrastructure features of systemic amyloidosis by using light microscope（LM） and transmission electron microscope（TEM）.Methods： The biopsy specimens of kidney,adipose tissue of abdominal wall and rectal mucous membrane were obtained from 21 patients with systemic amyloidosis and processed by routine HE and Congo red stain for LM and thinsection for EM.Results： Under LM,the percentages of positive amyloid deposits in the biopsy specimens of kidney,adipose tissue of abdominal wall and rectal mucous membrane were 76.2%（16/21）、38.1%（8/21）and 9.5%（2/21）respectively.Under TEM,the amyloid deposits have the moderate-density,nonuniform cottony appearance.The amyloid fibrils are relatively straight,nonbranching,without granules and arranged reticulately and about 10 nm in diameter.The percentages of positive amyloid fibrils found in specimens of kidney,adipose tissue of abdominal wall and rectal mucous membrane were 90.5%（19/21）、66.7%（14/21）and 42.9%（9/21） respectively.Conclusion： Electron microscopic examination is an important aid in the ultrapathological diagnosis of systemic amyloidosis,especially in the early stage.The biopsy of both adipose tissue of abdominal wall and rectal mucous membrane is useful and safe in the diagnosis of systemic amyloidosis as well.