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| 柳树叶中原花青素含量测定 | |
| 引用本文： | 陈洪玉,迟彩霞,王德新,王佩华. 柳树叶中原花青素含量测定[J]. 陕西化工, 2012, 0(8): 1456-1457,1461 |
| 作者姓名： | 陈洪玉 迟彩霞 王德新 王佩华 |
| 作者单位： | 绥化学院食品与制药工程学院,黑龙江绥化152061 |
| 基金项目： | 绥化学院科学研究项目（K1102002） |
| 摘 要： | 采用紫外．可见分光光度法测定柳树叶中的原花青素的含量，酸化香草醛比色法，测定波长为500nm，浓盐酸1．5mL，4％香草醛3mL，反应时间为20min。结果表明，该方法的线性范围为4．0—100．0μg/mL，相关系数R^2=0．9991，平均加标回收率为94．87％，相对标准偏差（RSD）1．38％。该法操作简便、准确、稳定，适用于原花青素含量的测定。 |
| 关 键 词： | 柳树叶 原花青素 含量测定 |
Determination of procyanidins content of willow leaves |
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| CHEN Hong-yu,CHI Cai-xia,WANG De-xin,WANG Pei-hua. Determination of procyanidins content of willow leaves[J]. Applied Chemical Industry, 2012, 0(8): 1456-1457,1461 | |
| Authors: | CHEN Hong-yu CHI Cai-xia WANG De-xin WANG Pei-hua |
| Affiliation: | ( Food and Pharmaceutical Engineering College, Suihua College, Suihua 152061, China) |
| Abstract: | Spectrophotometric method was used for analysis of proanthocyanidin from willow leaves. Vanil- lin assay was carried out, under the conditions of wavelength at 500 nm, concentrated hydrochloric acid 1.5 mL,4% vanillin 3 mL, reaction time 20 min. The linear range of 4.0 -100.0 μg/mL, the correlation coefficient was R^2 =0. 999 1 ,the average recovery of standard addition was 94.87%, RSD was 1.38%. This method is simple, accurate, stable and can be used for assay of procyanidins. |
| Keywords: | willow leaves proanthocyanidins content determination |
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