不同培养基及培养条件对单增李斯特菌ERIC-PCR分子分型的影响

本研究以实验室保存的26株李斯特氏菌为实验对象,通过ERIC-PCR和凝胶电泳技术对其展开基因分型,并根据分型结果选取不同亚型菌株,进而考虑改变细菌培养条件来探究不同培养基对李斯特菌ERIC-PCR分子分型结果的影响。实验结果初步表明,实验室26株李斯特菌至少可分成11个主要基因类群,其中Ⅰ型菌株最多占有8株,而Ⅲ、Ⅶ、Ⅷ、Ⅸ、Ⅹ以及Ⅺ型均最少,各占1株;同时发现,培养基及培养条件的改变会对LM87、LM109(野生型单增李斯特菌)分型结果产生影响,且这些菌株分型均最少。由此推断,不同的营养机制和培养条件可能会对李斯特菌的基因组稳定性或基因表达带来潜在影响,因此无论是在对LM致病机理的基础研究过程中还是对其进行分子分型鉴定,都必须考虑其寄主来源以及培养条件。

Influence of Different Medium Conditions on Molecular Typing of Listeria monocytogenes by Enterobacterial Repetitive Intergenic Consensus PCR

Twenty-six strains of Listeria spp. stored in the laboratory were used as the experimental object here, and the enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) and gel electrophoresis techniques were applied to genotyping. Furthermore, according to the typing results, strains of different subtypes were selected, and the effects of different medium conditions on the results of ERIC-PCR molecular typing of Listeria spp. were explored by changing the culture conditions. The preliminary test results showed that 26 strains of Listeria spp. in the laboratory could be subdivided into at least 11 genotypes, and the number of the strains belonging to genotype I was the highest, whereas the numbers of strains belonging to genotypes III, VII, VIII, IX, X, and XI were the lowest. In addition, the results revealed that the changes in medium and culture conditions affected the typing results of LM89 and LM109 (wild-type Listeria monocytogenes), which belong to the genotypes with the lowest number of strains. From this preliminary study, it can be concluded that different nutritional conditions and culture conditions may have an impact on the genomic stability or gene expression of Listeria spp. Therefore, the host source and culture conditions must be taken into account during basic research on L. monocytogenes pathogenicity and its molecular typing.