液相色谱-电喷雾离子阱质谱测定烟草中的游离茄尼醇

用液相色谱-电喷雾离子阱质谱(LC/ESI-ITMS)建立了-种灵敏、快速可靠的分析烟草游离茄尼醇的方法.烟草样品用85%甲醇-15%异丙醇(V/V)超声萃取30min,在分析前无需进行其他前处理.在1.8μm快速分离C<,18>色谱短柱上用85%甲醇-15%异丙醇作为流动相等梯度洗脱实现了茄尼醇的快速分离.在电喷雾正...

Determination of Free Solanesol in Tobacco by Liquid Chromatography and Electrosoray Ionization Ion Trap Mass Spectrometry

A rapid,sensitive and reliable liquid chromatography / electrospray ionization-ion trap mass spectrometry(LC/ESI-ITMS) method was developed for the determination of free solanesol in tobacco.Solanesol was efficiently extracted by ultrasonic extraction from tobacco with methanol?isopropanol(85:15,V/V) for 30 min and no further workup procedure was needed before analyzed.Solanesol was separated by using a short 1.8 ?m rapid separation C18 column with methanol?isopropanol(85:15,V/V) as the mobile phase in the isocratic gradient to achieve a fast chromatographic analysis.In the positive ESI mode,the sodium adduct ion(+,m/z 653.8) selected for quantitation was sensitively detected in the two-stage mass(MS2) full scan mode without employing collision energy.The limit of detection(LOD) was 5 ng/mL.The intra-day and the inter-day precision were 3.7% and 5.0%,respectively.The recoveries were 96% and 102% for two different spiked levels.The established method has been successfully applied to the determination of solanesol in various tobacco samples.