| 时间分辨免疫荧光法测定食物中花生过敏原成分 |
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| 引用本文： | 刘志刚,李佳娜,顾耀亮,夏立新,陈家杰.时间分辨免疫荧光法测定食物中花生过敏原成分[J].食品科技,2010(2). |
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| 作者姓名： | 刘志刚 李佳娜 顾耀亮 夏立新 陈家杰 |
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| 作者单位： | 深圳大学过敏反应与免疫学研究所;中山火炬职业技术学院生物医药系; |
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| 基金项目： | 国家自然科学基金项目(30871752); 深港创新圈项目(2007) |
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| 摘 要： | 目的:建立双抗体夹心时间分辨荧光免疫法Time-resolved Fluoroimmunoassay(TRFIA)]测定食物中花生过敏原蛋白成分,为进出口食品中花生过敏原成分检测和由花生导致的食物过敏性疾病的预防提供技术基础。方法:提取花生蛋白,免疫小鼠制备抗花生总蛋白的多克隆抗体,用该抗体包被酶标板,并用生物素标记该多抗作为捕捉抗体,Eu3+标记的链酶亲和素和以β-二酮体为主的增强液等试剂,建立双抗夹心TRFIA方法,检测该方法的灵敏度,同时用于13种食品中花生过敏原蛋白成分检测。结果:初步地研制出双抗体夹心TRFIA法检测食品中花生过敏原蛋白成分,具有特异性,和其最低检出限为0.1ng/mL,标准曲线在0.1～160ng/mL范围内线性良好;11种食品检测结果与食物过敏原标签标注内容相符,而2种标示不详的食品检测结果均呈现阳性。
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| 关 键 词： | 时间分辨荧光免疫法 花生过敏原 多克隆抗体 食物过敏 |
Determination of peanut allergens protein trace in food products by time-resolved fluoroimmunoassay |
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| LIU Zhi-gang,LI Jia-na,GU Yao-liang,XIA Li-xin,CHEN Jia-jie.Determination of peanut allergens protein trace in food products by time-resolved fluoroimmunoassay[J].Food Science and Technology,2010(2). |
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| Authors: | LIU Zhi-gang LI Jia-na GU Yao-liang XIA Li-xin CHEN Jia-jie |
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| Affiliation: | LIU Zhi-gang1,LI Jia-na1,GU Yao-liang2,XIA Li-xin1,CHEN Jia-jie1 (1.Allergy , Immunology Institute,Shenzhen University,Shenzhen 518060,2.Department of Biomedicine,Zhongshan Torch Polytechnic,Zhongshan 528437) |
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| Abstract: | Objective: To develop a new method with sandwich-antibody time-resolved fluoroimmunoassay (TRFIA) to detect peanut allergens protein trace in food products, which will provide a technical tool to prevent peanut allergy. Method: The peanut proteins were extracted and further analyzed by SDS-PAGE. The Balb/c mice were immuned with the extracts of peanut proteins and the highly-titrated polyclonal IgG antibodies against them were isolated and coupled with biotin for TRFIA kit. Thirteen food products were teste... |
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| Keywords: | TRFIA peanut allergen polyclonal antibody food allergy |
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