| 树状大分子包裹的金纳米颗粒对PCR优化的影响 |
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| 引用本文： | 陈晶晶,曹雪雁,郭睿,史向阳.树状大分子包裹的金纳米颗粒对PCR优化的影响[J].纳米科技,2011(1):29-33,45. |
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| 作者姓名： | 陈晶晶 曹雪雁 郭睿 史向阳 |
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| 作者单位： | 东华大学化学化工与生物工程学院,上海201620 |
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| 基金项目： | supported by the Shanghai Pujiang Program （09PJ1400600）; the National Basic Research Program of China （973 Program 2007CB936000）; the Program for Professor of Special Appointment （Eastern Scholar） at Shanghai Institutions of Higher Learning; the Fundamental Research Funds for the Central Universities （For X. C. R. G. and X. S.）; the Young Teacher Foundation of Donghua University （2009）; supports from Shanghai Natural Science Foundation （10ZR1400800） |
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| 摘 要： | 以283bp的λDNA的再扩增PCR体系为测试体系,对树状大分子包裹的金纳米颗粒对PCR的作用进行研究,实验证明,包裹有不同浓度胶体金的树状大分子都能对PCR反应起到优化作用,而且随着胶体金浓度的增大,相应树状大分子的优化作用增强,最优浓度越来越低,结果表明,树状大分子包裹的金纳米颗粒的优化机制是胶体金的加入,使树状大分子的＂硬度＂增大,当树状大分子与PCR各组分相互作用时,由于其表面的有效反应基团增多,提高周围PCR各组分有效反应浓度,从而增强反应的效率及特异性。
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| 关 键 词： | 树状大分子包裹的金纳米颗粒 PCR 特异性 效率 |
Effect of Dendrimer-entrapped Gold Nanoparticles on the Polymerase Chain Reaction |
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| Authors: | CHEN Jing-jing CAO Xue-yan GUO Rui SHI Xiang-yang |
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| Affiliation: | (College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China) |
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| Abstract: | The use of dendrimer-entrapped gold nanoparticles （Au DENPs） as a novel class of enhancers to improve the specificity and efficiency of PCR was reported. The experiment results show that the Au DENPs were effective in optimizing error-prone two-round PCR in specificity and efficiency. With the increasing of molar ratio between gold atom and amine-terminated generation 5 poly（amidoamine） dendrimer （G5.NH2） in the Au DENPs, the optimum concentration of Au DENPs could be lower and lower. The present study suggests that the molecular mechanism of the enhancing effect of the dendrimer on PCR is presumably due to the interaction of PCR components with the amine groups on the surface of the dendrimers. |
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| Keywords: | tree macromolecular package gold nanoparticles PCR specificity efficiency |
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