马钱苷的电喷雾串联质谱及傅立叶变换离子回旋共振质谱研究

利用电喷雾串联质谱（ESI-MSⁿ）技术研究马钱苷的质谱碎裂机理, 探讨其碎裂方式，并利用傅立叶变换离子回旋共振质谱（FTICR-MS）及SORI-CID高分辨率、高质量准确度对其碎裂机理进行验证研究。采用 H/D 交换和串联质谱的方法，发现马钱苷羟基上的活泼氢在质谱中性碎片丢失过程中较易被转移，表现出较大的化学活性。通过加入特定的Na⁺ 和 Li⁺ 等碱金属离子，极大地提高了马钱苷化合物质谱分析的灵敏度，获得了更多的结构信息，建立了马钱苷化合物质谱鉴定和分析的新方法。

Study on the Loganin by ESI-MSn and FTICR-MS

Loganin was investigated systematically by electrospray ionization tandem mass spectrometry (ESI-MSⁿ) to propose the logical fragmentation pathways. Product ion mass spectra examined at high mass resolution allowed unambiguous determination of the elemental composition of fragment ions by sustained off-resonance irradiation/collision induced dissociation (SORI-CID) method in fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS). Through hydrogen and deuterium exchange, the fragment path of Loganin is further confirmed. Experiment demonstrates that M+Li]⁺ and M+Na]⁺ ions can provide complementary useful structural information for structure analysis of Loganin.