| 烟草色素蛋白复合物中多酚类物质对其抗紫外性能的影响 |
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| 引用本文： | 林晓婕,傅红,杨琳,郑晨晖.烟草色素蛋白复合物中多酚类物质对其抗紫外性能的影响[J].中国烟草科学,2011,32(2):57-61. |
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| 作者姓名： | 林晓婕 傅红 杨琳 郑晨晖 |
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| 作者单位： | 1. 福州大学生物科学与工程学院,福州,350108
2. 福建省海洋环境与渔业资源监测中心,福州,350003 |
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| 摘 要： | 采用不同的方法获得烟草色素蛋白复合物(tobacco pigments protein,TPP),研究其抗紫外性能及机理。分别通过等电点沉降和硫酸铵沉降从烟草中分离得到TPP,运用高效液相色谱对其所结合的酚类物质进行了分析检测,并对其进行紫外可见图谱扫描,同时通过日光辐照,观察其降解速度,确定其抗紫外辐射活性。结果表明,多酚类物质如芸香苷、咖啡酸、绿原酸和莨菪葶,在抗紫外辐射过程中扮演重要角色;pH 4.5、pH 5.0、pH 5.3沉降的TPP的总酚酸含量高,其中pH 4.5沉降的TPP的酚类物质含量最高,抗紫外活性也最强;另外,85%硫酸铵沉降获得的TPP总酚酸含量低,抗紫外活性也较弱。在长时间的日光照射试验中,TPP的抗紫外性能表现稳定。
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| 关 键 词： | 烟草 色素蛋白复合物 抗紫外性能 多酚类物质 |
Effect of Phenols in Tobacco Pigments-protein Complex on Anti-uv Function |
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| LIN Xiaojie,FU Hong,YANG Lin,ZHENG Chenhui.Effect of Phenols in Tobacco Pigments-protein Complex on Anti-uv Function[J].Chinese Tobacco Science,2011,32(2):57-61. |
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| Authors: | LIN Xiaojie FU Hong YANG Lin ZHENG Chenhui |
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| Affiliation: | LIN Xiaojie1,FU Hong1*,YANG Lin2,ZHENG Chenhui1(1.College of Biological Science and Technology,Fuzhou University,Fuzhou 350108,China,2.Monitoring Center of Marine Environment and Fishery Resources,Fuzhou 350003,China) |
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| Abstract: | Anti-Ultraviolet properties and mechanism of pigments protein complex from tobacco leaves were studied.Proteins were precipitated by ammonium sulphate solution of 85% saturation or isoelectric point precipitation from tobacco leaves.The profiles of binding phenolic acids such as chlorogenic acid,rutin,scopoletin and caffeic acid were analyzed by High Performance Liquid Chromatography,the anti-UV function of pigments-protein were determined with UV spectrum after being irradiated by sunshine,and the speed of... |
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| Keywords: | tobacco pigments protein ultraviolet irradiation phenolics |
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