| 竹叶天然抗菌肽的分离纯化与抗菌活性研究 |
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| 引用本文： | 江惠,张国栋. 竹叶天然抗菌肽的分离纯化与抗菌活性研究[J]. 食品工业, 2011, 0(5) |
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| 作者姓名： | 江惠 张国栋 |
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| 作者单位： | 西华大学生物工程学院; |
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| 基金项目： | 四川省教育厅自然科学重点项目(2005A114) |
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| 摘 要： | 利用PBS浸提、硫酸铵梯度盐析、弱阴离子交换色谱DEAE-A50,从竹叶中分离纯化出一种新的抗菌肽。该活性多肽经纯化后在SDS-PAGE上显示为两条带,分子量分别为49 888 Da和45 604 Da。此竹叶多肽对大肠杆菌(Escherichia coli)、金黄色葡萄球菌(Staphylococcus aureus)以及枯草芽孢杆菌(Bacillus subtilis)表现出良好的抑菌活性。
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| 关 键 词： | 梯度盐析 竹叶 纯化 抑菌活性 |
Purification and Antimicrobial Activity of a Natural Antimicrobial Peptide from Bamboo Leaves |
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| Jiang Hui,Zhang Guo-dong College of Bio-Engineering,Xihua University. Purification and Antimicrobial Activity of a Natural Antimicrobial Peptide from Bamboo Leaves[J]. The Food Industry, 2011, 0(5) |
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| Authors: | Jiang Hui Zhang Guo-dong College of Bio-Engineering Xihua University |
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| Affiliation: | Jiang Hui,Zhang Guo-dong College of Bio-Engineering,Xihua University(Chengdu 610039) |
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| Abstract: | A natural antimicrobial peptide was isolated from bamboo leaves by extraction with PBS,gradient salting out,and purified by weak anionic exchange chromatograph column(DEAE-A50).It was identified to be two bandings by SDS-PAGE,and the relative molecular weights were estimated to be about 49888 Da and 45604 Da respectively.The purified antimicrobial peptide showed strong antimicrobial activity against Escherichia coli,Staphylococcus aureus and Bacillus subtilis. |
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| Keywords: | gradient salting out bamboo leaves purification antimicrobial activity |
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