| 汽巴蓝功能微球对人血清白蛋白的吸附性能研究 |
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| 引用本文： | 沈红燕,熊万斌,杨吉,龚雁,刘晓亚,陈明清. 汽巴蓝功能微球对人血清白蛋白的吸附性能研究[J]. 功能材料, 2009, 40(6) |
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| 作者姓名： | 沈红燕 熊万斌 杨吉 龚雁 刘晓亚 陈明清 |
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| 作者单位： | 江南大学,化学与材料工程学院,江苏,无锡,214122 |
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| 基金项目： | 国家自然科学基金资助项目(20671043); 江苏高等学校科技创新团队资助项目(苏教科[2007]5号) |
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| 摘 要： | 通过分散共聚制得了聚苯乙烯接枝聚醋酸乙烯酯(PSt-g-PVAc)微球,粒径控制在500～700nm之间.在碱性条件下将PSt-g-PVAc微球表面的PVAc链醇解为聚乙烯醇(PVA),得到了PSt-g-PVA微球,提高了其在水中的分散稳定性.使汽巴蓝(CB)与PSt-g-PVA表面的羟基进行亲核反应,制得了CB作为配体的新型吸附剂汽巴蓝功能微球(CB微球),元素分析测得CB微球表面的CB最大含量为139.22μmol/g,探讨了其在不同人血清白蛋白(HSA)浓度、pH值和吸附时间下对HSA的吸附性能.当HSA浓度为1.0mg/mL、pH为5.14时,CB微球对HSA的最大吸附量为40.9mg/g,并分析了CB微球与HSA的相互作用机理.由NaSCN进行解吸试验,发现可将吸附在CB微球上的HSA解吸92.11%以上,且CB微球的重复使用性能良好.
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| 关 键 词： | 人血清白蛋白 分散共聚 CB微球 吸附 |
Studies of human serum albumin adsorption on cibacron blue F3GA functional microspheres |
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| SHEN Hong-yan
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XIONG Wan-bin
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YANG Ji
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GONG Yan
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LIU Xiao-ya
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CHEN Ming-qing. Studies of human serum albumin adsorption on cibacron blue F3GA functional microspheres[J]. Journal of Functional Materials, 2009, 40(6) |
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| Authors: | SHEN Hong-yan
XIONG Wan-bin
YANG Ji
GONG Yan
LIU Xiao-ya
CHEN Ming-qing |
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| Affiliation: | SHEN Hong-yan,XIONG Wan-bin,YANG Ji,GONG Yan,LIU Xiao-ya,CHEN Ming-qing (School of Chemical and Material Engineering,Jiangnan University,Wuxi 214122,China) |
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| Abstract: | Polystyrene grafted poly(vinyl acetate) (PSt-g-PVAc) microspheres were obtained by dispersion copolymerization of PVAc macromonomer with styrene. The diameter of PSt-g-PVAc microspheres was controlled in range of 500-700nm. Polystyrene grafted poly(vinyl alcohol) (PSt-g-PVA) microspheres were derived from alkaline hydrolysis of PVAc chains on surfaces of PSt-g-PVAc microspheres in the presence of NaOH. Dispersion stability of the PSt-g-PVA microspheres was increased in water. The CB functional microspheres ... |
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| Keywords: | human serum albumin dispersion copolymerization CB functional microsphere adsorption |
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